Next Article in Journal
Plant-Derived Nanovesicles: A Comprehensive Review from Isolation to Clinical Translation—Unlocking Natural Nanocarriers for Biomedical Applications
Previous Article in Journal
A Liposomal Delivery System of Blueberry Anthocyanins Ameliorates Corneal Laser Injury
Previous Article in Special Issue
Mitochondrial ROS in Retinal Neurodegeneration: Thresholds, Quality Control Failure, and Precision Therapeutic Windows
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 16
Issue 5
10.3390/biom16050704
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Exploring Replicative Senescence and Oxidative Stress-Induced Remodelling of Mitochondrial-Associated Membranes in Human Skin Fibroblasts

by
Anne-Laure Bulteau
1,*,†,
Gallic Beauchef
1,†,
Stéphanie Chanon
2,
Aurélie Vieille-Marchis
2,
Julien Chlasta
3,
Gaël Runel
3,
Juliette Sage
1,
Tanesha Naiken
1,
Lauren Sobilo
1,
Elodie Bossard
1,
Lorene Gourguillon
1,
Carine Nizard
1,
Karl Pays
1,
Laurence Canaple
4 and
Beatrice Morio
2,*
1
LVMH Recherche, 185 Avenue de Verdun, 45800 Saint Jean de Braye, France
2
Laboratoire CarMeN, UMR INSERM U1060/INRAe U1397, Université Claude Bernard Lyon1, 69310 Pierre-Bénite, France
3
BIOMECA, 69008 Lyon, France
4
SFR Biosciences, AniRA-ImmOs, CNRS UAR 3444, Inserm US8, UCBL, ENS de Lyon, 69007 Lyon, France
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2026, 16(5), 704; https://doi.org/10.3390/biom16050704 (registering DOI)
Submission received: 25 March 2026 / Revised: 22 April 2026 / Accepted: 7 May 2026 / Published: 11 May 2026
(This article belongs to the Special Issue Mitochondrial ROS in Health and Disease: 2nd Edition)
Versions Notes

Abstract

(1) Background: Calcium transfer between the endoplasmic reticulum (ER) and mitochondria through the IP3R–VDAC1 complex at mitochondria-associated ER membranes (MAMs) is essential for cellular homeostasis. Alterations in this signalling axis have been implicated in ageing and cellular senescence. (2) Methods: We developed an in vitro human dermal fibroblast (HDF) model combining replicative senescence and acute oxidative stress to investigate the role of ER–mitochondria coupling in skin ageing and to enable biomolecule screening. (3) Results: In situ proximity ligation assays revealed that replicative senescence significantly increased the number of VDAC1/IP3R complexes per cell (+85% and +72%, p < 0.01), together with elevated cellular reactive oxygen species (+47% and +74%, p < 0.05). Consistently, acute oxidative stress (50 µM t-BHP, 30 min) rapidly increased VDAC1/IP3R complexes (+48%, p < 0.001) and intra-mitochondrial calcium levels (+19%, p < 0.001). These effects persisted for 24 h post-treatment and were associated with impaired mitochondrial function (−27% in the Bioenergetic Health Index, p < 0.05). We also established a flexibility index capturing both acute and long-term adaptations and detecting the protective effects of an orchid extract. (4) Conclusions: ER–mitochondria coupling disruption via the IP3R–VDAC1 complex may contribute to oxidative stress-induced senescence and represent a key mechanism in extrinsic skin ageing.
Keywords: skin ageing; mitochondria-ER contact sites; oxidative stress; intra-mitochondrial calcium; Bioenergetic Health Index; cytoskeleton physical properties skin ageing; mitochondria-ER contact sites; oxidative stress; intra-mitochondrial calcium; Bioenergetic Health Index; cytoskeleton physical properties

Share and Cite

MDPI and ACS Style

Bulteau, A.-L.; Beauchef, G.; Chanon, S.; Vieille-Marchis, A.; Chlasta, J.; Runel, G.; Sage, J.; Naiken, T.; Sobilo, L.; Bossard, E.; et al. Exploring Replicative Senescence and Oxidative Stress-Induced Remodelling of Mitochondrial-Associated Membranes in Human Skin Fibroblasts. Biomolecules 2026, 16, 704. https://doi.org/10.3390/biom16050704

AMA Style

Bulteau A-L, Beauchef G, Chanon S, Vieille-Marchis A, Chlasta J, Runel G, Sage J, Naiken T, Sobilo L, Bossard E, et al. Exploring Replicative Senescence and Oxidative Stress-Induced Remodelling of Mitochondrial-Associated Membranes in Human Skin Fibroblasts. Biomolecules. 2026; 16(5):704. https://doi.org/10.3390/biom16050704

Chicago/Turabian Style

Bulteau, Anne-Laure, Gallic Beauchef, Stéphanie Chanon, Aurélie Vieille-Marchis, Julien Chlasta, Gaël Runel, Juliette Sage, Tanesha Naiken, Lauren Sobilo, Elodie Bossard, and et al. 2026. "Exploring Replicative Senescence and Oxidative Stress-Induced Remodelling of Mitochondrial-Associated Membranes in Human Skin Fibroblasts" Biomolecules 16, no. 5: 704. https://doi.org/10.3390/biom16050704

APA Style

Bulteau, A.-L., Beauchef, G., Chanon, S., Vieille-Marchis, A., Chlasta, J., Runel, G., Sage, J., Naiken, T., Sobilo, L., Bossard, E., Gourguillon, L., Nizard, C., Pays, K., Canaple, L., & Morio, B. (2026). Exploring Replicative Senescence and Oxidative Stress-Induced Remodelling of Mitochondrial-Associated Membranes in Human Skin Fibroblasts. Biomolecules, 16(5), 704. https://doi.org/10.3390/biom16050704

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop