Cannabinoid Regulation of Murine Vaginal Secretion
Abstract
:1. Introduction
2. Methods
2.1. Study Animals
2.2. Experimental Design
2.2.1. Sex and Disposition of Animals
2.2.2. Number of Mice Used for Each Experiment
- (1)
- THC, IP 4 mg/kg, active phase, n = 16;
- (2)
- THC, IP 10 mg/kg, active phase, n = 8;
- (3)
- THC, IP 4 mg/kg, rest phase, n = 8;
- (4)
- THC, Intravaginal (IVag) 1 mM (10 μL), n = 9;
- (5)
- CP55940, IP 0.5 mg/kg, n = 10;
- (6)
- CP55940, IntraVag 150 μM (10 μL), n = 13;
- (7)
- SR141716, IP 4 mg/kg, n = 12.
- (1)
- CP55940, IP 0.5 mg/kg, n = 12;
- (2)
- Vehicle, IP, n = 13;
- (3)
- CP55940, IVag 150 μM (10 μL), n = 30.
- (1)
- CB1 KO vs. WT (CD1 strain) baselines, n = 30, 32;
- (2)
- CB1 KO bedding responses, n = 16;
- (3)
- WT, SR141716, IP 4 mg/kg, n = 24;
- (4)
- WT, SR141716, IVag 1 mM (10 μL), n = 15.
- (1)
- THC, IP 4 mg/kg, daily, n = 10;
- (2)
- THC, IP 4 mg/kg, chronic (weekly), n = 16.
- (1)
- CP55940, IP 0.5 mg/kg (daily), CD1 strain, n = 13;
- (2)
- CP55940, IP 0.5 mg/kg (weekly), CD1 strain, n = 7;
- (3)
- CP55940, IP 0.5 mg/kg (weekly), CB1 knockout on CD1 strain, n = 8.
2.2.3. Measurement of Vaginal Moisture
2.3. Bedding
2.3.1. General Method of Exposure to Odorant (Bedding)
2.3.2. Chronic Treatments
2.4. Statistics
2.5. Drugs
3. Results
3.1. Effects of Acute Treatment with THC, CP55940, and SR141716 on Basal Vaginal Moisture
3.2. Effect of CP55940 on Stimulated Vaginal Secretion
3.3. Effect of CB1 Receptor Block or Deletion on Stimulated Vaginal Secretion
3.4. Chronic THC Does Not Alter Vaginal Moisture Levels
3.5. Chronic Intermittent CP55940 Reduces Basal Vaginal Moisture and Unveils a Stimulatory CB1-Mediated Effect
4. Discussion
4.1. An Effect on Stimulated but Not Basal Vaginal Moisture
4.2. The Mouse as a Model
4.3. Potential Mechanisms
4.4. THC, CP55940, and the Potential Effects of Cannabis
4.5. Ruling out the Role of Olfactory Cannabinoid Receptors
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Murataeva, N.; Mattox, S.; Yust, K.; Straiker, A. Cannabinoid Regulation of Murine Vaginal Secretion. Biomolecules 2025, 15, 472. https://doi.org/10.3390/biom15040472
Murataeva N, Mattox S, Yust K, Straiker A. Cannabinoid Regulation of Murine Vaginal Secretion. Biomolecules. 2025; 15(4):472. https://doi.org/10.3390/biom15040472
Chicago/Turabian StyleMurataeva, Natalia, Sam Mattox, Kyle Yust, and Alex Straiker. 2025. "Cannabinoid Regulation of Murine Vaginal Secretion" Biomolecules 15, no. 4: 472. https://doi.org/10.3390/biom15040472
APA StyleMurataeva, N., Mattox, S., Yust, K., & Straiker, A. (2025). Cannabinoid Regulation of Murine Vaginal Secretion. Biomolecules, 15(4), 472. https://doi.org/10.3390/biom15040472