Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen

Tamaki, Tetsuro; Natsume, Toshiharu; Katoh, Akira; Shigenari, Atsuko; Shiina, Takashi; Nakajima, Nobuyuki; Saito, Kosuke; Fukuzawa, Tsuyoshi; Otake, Masayoshi; Enya, Satoko; Kangawa, Akihisa; Imai, Takeshi; Tamaki, Miyu; Uchiyama, Yoshiyasu

doi:10.3390/biom14080939

Open AccessArticle

Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen

by

Tetsuro Tamaki

^1,2,*

,

Toshiharu Natsume

^1,2,

Akira Katoh

^1,2

,

Atsuko Shigenari

³,

Takashi Shiina

³,

Nobuyuki Nakajima

^1,4

,

Kosuke Saito

^1,5,

Tsuyoshi Fukuzawa

^1,6,

Masayoshi Otake

⁷,

Satoko Enya

⁷,

Akihisa Kangawa

⁷

,

Takeshi Imai

^1,8,

Miyu Tamaki

^1,8 and

Yoshiyasu Uchiyama

^1,8

¹

Muscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan

²

Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

³

Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

⁴

Department of Urology, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

⁵

Department of Otolaryngology, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

⁶

Department of Radiation Oncology, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

⁷

Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Kikugawa 439-0037, Shizuoka, Japan

⁸

Department of Orthopedic Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Japan

^*

Author to whom correspondence should be addressed.

Biomolecules 2024, 14(8), 939; https://doi.org/10.3390/biom14080939

Submission received: 21 May 2024 / Revised: 28 July 2024 / Accepted: 29 July 2024 / Published: 2 August 2024

(This article belongs to the Special Issue Recent Developments in Mesenchymal Stem Cells)

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Abstract

Pig skeletal muscle-derived stem cells (SK-MSCs) were transplanted onto the common peroneal nerve with a collagen tube as a preclinical large animal experiment designed to address long nerve gaps. In terms of therapeutic usefulness, a human family case was simulated by adjusting the major histocompatibility complex to 50% and 100% correspondences. Swine leukocyte antigen (SLA) class I haplotypes were analyzed and clarified, as well as cell transplantation. Skeletal muscle-derived CD34+/45− (Sk-34) cells were injected into bridged tubes in two groups (50% and 100%) and with non-cell groups. Therapeutic effects were evaluated using sedentary/general behavior-based functional recovery score, muscle atrophy ratio, and immunohistochemistry. The results indicated that a two-Sk-34-cell-transplantation group showed clearly and significantly favorable functional recovery compared to a non-cell bridging-only group. Supporting functional recovery, the morphological reconstitution of the axons, endoneurium, and perineurium was predominantly evident in the transplanted groups. Thus, Sk-34 cell transplantation is effective for the regeneration of peripheral nerve gap injury. Additionally, 50% and 100% SLA correspondences were therapeutically similar and not problematic, and no adverse reaction was found in the 50% group. Therefore, the immunological response to Sk-MSCs is considered relatively low. The possibility of the Sk-MSC transplantation therapy may extend to the family members beyond the autologous transplantation.

Keywords: preclinical large animal experiment; micro-mini pig; cloning; allogeneic cellular therapy; swine leukocyte antigen haplotypes

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MDPI and ACS Style

Tamaki, T.; Natsume, T.; Katoh, A.; Shigenari, A.; Shiina, T.; Nakajima, N.; Saito, K.; Fukuzawa, T.; Otake, M.; Enya, S.; et al. Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen. Biomolecules 2024, 14, 939. https://doi.org/10.3390/biom14080939

AMA Style

Tamaki T, Natsume T, Katoh A, Shigenari A, Shiina T, Nakajima N, Saito K, Fukuzawa T, Otake M, Enya S, et al. Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen. Biomolecules. 2024; 14(8):939. https://doi.org/10.3390/biom14080939

Chicago/Turabian Style

Tamaki, Tetsuro, Toshiharu Natsume, Akira Katoh, Atsuko Shigenari, Takashi Shiina, Nobuyuki Nakajima, Kosuke Saito, Tsuyoshi Fukuzawa, Masayoshi Otake, Satoko Enya, and et al. 2024. "Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen" Biomolecules 14, no. 8: 939. https://doi.org/10.3390/biom14080939

APA Style

Tamaki, T., Natsume, T., Katoh, A., Shigenari, A., Shiina, T., Nakajima, N., Saito, K., Fukuzawa, T., Otake, M., Enya, S., Kangawa, A., Imai, T., Tamaki, M., & Uchiyama, Y. (2024). Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen. Biomolecules, 14(8), 939. https://doi.org/10.3390/biom14080939

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Skeletal Muscle-Derived Stem Cell Transplantation Accelerates the Recovery of Peripheral Nerve Gap Injury under 50% and 100% Allogeneic Compatibility with the Swine Leucocyte Antigen

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