[18F]GE-180-PET and Post Mortem Marker Characteristics of Long-Term High-Fat-Diet-Induced Chronic Neuroinflammation in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Model
2.2. PET/CT Imaging and Image Analysis
2.3. Behavioural Test: Morris Water Maze
2.4. Weight Control, Blood Sampling, Euthanasia and Tissue Preparation
2.5. Blood and Plasma Analyses
2.6. Histology and Immunohistochemistry
2.7. Quantitative Real-Time PCR
2.8. Statistical Analysis
3. Results
3.1. Long-Term High-Fat Diet Induces an Obese Phenotype with Brain Glucose Hypermetabolism
3.2. Long-Term High-Fat Diet Causes Only a Change in Pro-Inflammatory Cytokine Profile
3.3. Spatial Memory Function Is Unaffected by Long-Term High-Fat Diet-Derived Changes in the Brain
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Orientation | Sequence |
---|---|---|
rps18 | Forward Reverse | 5′-AGGATGTGAAGGATGGGAAG-3′ 5′-TTGGATACACCCACAGTTCG-3′ |
il-1β | Forward Reverse | 5′-CCCAAGCAATACCCAAAGAA-3′ 5′-TTGTGAGGTGCTGATGTACCA-3′ |
il-6 | Forward Reverse | 5′-GTTCTCTGGGAAATCGTGGA-3′ 5′-GGAAATTGGGGTAGGAAGGA-3′ |
tnfα | Forward Reverse | 5′-ACATTCGAGGCTCCAGTGAATTCGG-3′ 5′-GGCAGGTCTACTTTGGAGTCATTGC-3′ |
gfap | Forward Reverse | 5′-AGAAAACCGCATCACCATTC-3′ 5′-TCACATCACCACGTCCTTGT-3′ |
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Müller, L.; Power Guerra, N.; Schildt, A.; Lindner, T.; Stenzel, J.; Behrangi, N.; Bergner, C.; Alberts, T.; Bühler, D.; Kurth, J.; et al. [18F]GE-180-PET and Post Mortem Marker Characteristics of Long-Term High-Fat-Diet-Induced Chronic Neuroinflammation in Mice. Biomolecules 2023, 13, 769. https://doi.org/10.3390/biom13050769
Müller L, Power Guerra N, Schildt A, Lindner T, Stenzel J, Behrangi N, Bergner C, Alberts T, Bühler D, Kurth J, et al. [18F]GE-180-PET and Post Mortem Marker Characteristics of Long-Term High-Fat-Diet-Induced Chronic Neuroinflammation in Mice. Biomolecules. 2023; 13(5):769. https://doi.org/10.3390/biom13050769
Chicago/Turabian StyleMüller, Luisa, Nicole Power Guerra, Anna Schildt, Tobias Lindner, Jan Stenzel, Newshan Behrangi, Carina Bergner, Teresa Alberts, Daniel Bühler, Jens Kurth, and et al. 2023. "[18F]GE-180-PET and Post Mortem Marker Characteristics of Long-Term High-Fat-Diet-Induced Chronic Neuroinflammation in Mice" Biomolecules 13, no. 5: 769. https://doi.org/10.3390/biom13050769