Next Article in Journal
Recent Advances in Effector Research of Magnaporthe oryzae
Previous Article in Journal
Proteome Profiling of the Dystrophic mdx Mice Diaphragm
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 13
Issue 11
10.3390/biom13111649
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Gibberellic Acid Inhibits Dendrobium nobile—Piriformospora Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes

by
Hong Chen
1,2,
Yefei Li
1,3,
Yuying Yin
1,3,
Ji Li
1,2,
Lin Li
1,
Kunlin Wu
1,
Lin Fang
1,4,* and
Songjun Zeng
1,4,*
1
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
Department of Botany, Guangzhou Institute of Forestry and Landscape Architecture, Huangzhuang South Road 6, Baiyun District, Guangzhou 510540, China
3
University of the Chinese Academy of Sciences, Beijing 100049, China
4
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
*
Authors to whom correspondence should be addressed.
Biomolecules 2023, 13(11), 1649; https://doi.org/10.3390/biom13111649
Submission received: 30 August 2023 / Revised: 26 October 2023 / Accepted: 6 November 2023 / Published: 14 November 2023
Browse Figures
Review Reports Versions Notes

Abstract

Orchid seeds lack endosperms and depend on mycorrhizal fungi for germination and nutrition acquisition under natural conditions. Piriformospora indica is a mycorrhizal fungus that promotes seed germination and seedling development in epiphytic orchids, such as Dendrobium nobile. To understand the impact of P. indica on D. nobile seed germination, we examined endogenous hormone levels by using liquid chromatography–mass spectrometry. We performed transcriptomic analysis of D. nobile protocorm at two developmental stages under asymbiotic germination (AG) and symbiotic germination (SG) conditions. The result showed that the level of endogenous IAA in the SG protocorm treatments was significantly higher than that in the AG protocorm treatments. Meanwhile, GA3 was only detected in the SG protocorm stages. IAA and GA synthesis and signaling genes were upregulated in the SG protocorm stages. Exogenous GA3 application inhibited fungal colonization inside the protocorm, and a GA biosynthesis inhibitor (PAC) promoted fungal colonization. Furthermore, we found that PAC prevented fungal hyphae collapse and degeneration in the protocorm, and differentially expressed genes related to cell wall metabolism were identified between the SG and AG protocorm stages. Exogenous GA3 upregulated SRC2 and LRX4 expression, leading to decreased fungal colonization. Meanwhile, GA inhibitors upregulated EXP6, EXB16, and EXP10-2 expression, leading to increased fungal colonization. Our findings suggest that GA regulates the expression of cell wall metabolism genes in D. nobile, thereby inhibiting the establishment of mycorrhizal symbiosis.
Keywords: protocorm; symbiosis; cell wall; hormones protocorm; symbiosis; cell wall; hormones

Share and Cite

MDPI and ACS Style

Chen, H.; Li, Y.; Yin, Y.; Li, J.; Li, L.; Wu, K.; Fang, L.; Zeng, S. Gibberellic Acid Inhibits Dendrobium nobile—Piriformospora Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes. Biomolecules 2023, 13, 1649. https://doi.org/10.3390/biom13111649

AMA Style

Chen H, Li Y, Yin Y, Li J, Li L, Wu K, Fang L, Zeng S. Gibberellic Acid Inhibits Dendrobium nobile—Piriformospora Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes. Biomolecules. 2023; 13(11):1649. https://doi.org/10.3390/biom13111649

Chicago/Turabian Style

Chen, Hong, Yefei Li, Yuying Yin, Ji Li, Lin Li, Kunlin Wu, Lin Fang, and Songjun Zeng. 2023. "Gibberellic Acid Inhibits Dendrobium nobile—Piriformospora Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes" Biomolecules 13, no. 11: 1649. https://doi.org/10.3390/biom13111649

APA Style

Chen, H., Li, Y., Yin, Y., Li, J., Li, L., Wu, K., Fang, L., & Zeng, S. (2023). Gibberellic Acid Inhibits Dendrobium nobile—Piriformospora Symbiosis by Regulating the Expression of Cell Wall Metabolism Genes. Biomolecules, 13(11), 1649. https://doi.org/10.3390/biom13111649

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop