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Article

Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome

1
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
2
Department of Biology, University of Massachusetts Boston, Integrated Sciences Complex, 100 Morrissey Boulevard, Boston, MA 02125, USA
3
Department of Analytical Chemistry, University of Vienna, 1090 Vienna, Austria
*
Authors to whom correspondence should be addressed.
Current address: F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA.
Current address: Centogene GmbH, 18055 Rostock, Germany.
Academic Editor: Vladimir N. Uversky
Biomolecules 2022, 12(8), 1083; https://doi.org/10.3390/biom12081083
Received: 14 July 2022 / Revised: 1 August 2022 / Accepted: 3 August 2022 / Published: 6 August 2022
(This article belongs to the Special Issue Invertebrates as Emerging Model Organisms in Nutrition Research)
The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated. View Full-Text
Keywords: Drosophila; retina; vitamin A; retinal; proteome; lipidome; phototransduction; mitochondrion Drosophila; retina; vitamin A; retinal; proteome; lipidome; phototransduction; mitochondrion
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MDPI and ACS Style

Kumar, M.; Has, C.; Lam-Kamath, K.; Ayciriex, S.; Dewett, D.; Bashir, M.; Poupault, C.; Schuhmann, K.; Knittelfelder, O.; Raghuraman, B.K.; Ahrends, R.; Rister, J.; Shevchenko, A. Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome. Biomolecules 2022, 12, 1083. https://doi.org/10.3390/biom12081083

AMA Style

Kumar M, Has C, Lam-Kamath K, Ayciriex S, Dewett D, Bashir M, Poupault C, Schuhmann K, Knittelfelder O, Raghuraman BK, Ahrends R, Rister J, Shevchenko A. Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome. Biomolecules. 2022; 12(8):1083. https://doi.org/10.3390/biom12081083

Chicago/Turabian Style

Kumar, Mukesh, Canan Has, Khanh Lam-Kamath, Sophie Ayciriex, Deepshe Dewett, Mhamed Bashir, Clara Poupault, Kai Schuhmann, Oskar Knittelfelder, Bharath Kumar Raghuraman, Robert Ahrends, Jens Rister, and Andrej Shevchenko. 2022. "Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome" Biomolecules 12, no. 8: 1083. https://doi.org/10.3390/biom12081083

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