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Biomolecules
Volume 12
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10.3390/biom12071002
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Article

High-Throughput Proteomic Analysis of Human Dermal Fibroblast Response to Different Blood Derivatives: Autologous Topical Serum Derived from Plasma Rich in Growth Factors (PRGF) versus Leukocyte- and Platelet-Rich Plasma (L-PRP)

by
Eduardo Anitua
1,2,*,
Ander Pino
1,2,
Mikel Azkargorta
3,
Felix Elortza
3 and
Roberto Prado
1,2
1
BTI—Biotechnology Institute I MAS D, 01007 Vitoria, Spain
2
University Institute for Regenerative Medicine and Oral Implantology—UIRMI (UPV/EHU-Fundación Eduardo Anitua), 01007 Vitoria, Spain
3
Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII, Bizkaia Science and Technology Park, 48160 Derio, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2022, 12(7), 1002; https://doi.org/10.3390/biom12071002
Submission received: 15 June 2022 / Revised: 6 July 2022 / Accepted: 15 July 2022 / Published: 19 July 2022
(This article belongs to the Special Issue Novel Materials for Biomedical Applications)
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Abstract

Platelet-rich plasma (PRP) is nowadays used in the treatment of different types of cutaneous lesions. However, different compositions can influence clinical outcomes. Among them, the inclusion of leukocytes is controversial. High-throughput proteomics techniques were used to analyze the proteins that are differentially expressed in human dermal fibroblasts (HDFs) after treatment for 24 h with two PRP types, autologous topical serum (Endoret serum—ES) derived from plasma rich in growth factors (PRGF) and leukocyte- and platelet-rich plasma (L-PRP). The identified proteins were then classified by both Gene Ontology and Ingenuity Pathway Analysis. The obtained results show that the compositions of ES and L-PRP differ in such a way that they induce different responses in HDFs. ES-treated HDFs overexpress growth factor-related proteins, leading to protein synthesis, cell proliferation and migration. By contrast, L-PRP treatment induces a response similar to that caused by proinflammatory molecules. These data could explain the contradictory clinical results obtained for the different types of PRP, especially with respect to their leukocyte contents.
Keywords: platelet-rich plasma; PRP; plasma rich in growth factors; PRGF; topical serum; proteomics; regenerative medicine; skin platelet-rich plasma; PRP; plasma rich in growth factors; PRGF; topical serum; proteomics; regenerative medicine; skin

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MDPI and ACS Style

Anitua, E.; Pino, A.; Azkargorta, M.; Elortza, F.; Prado, R. High-Throughput Proteomic Analysis of Human Dermal Fibroblast Response to Different Blood Derivatives: Autologous Topical Serum Derived from Plasma Rich in Growth Factors (PRGF) versus Leukocyte- and Platelet-Rich Plasma (L-PRP). Biomolecules 2022, 12, 1002. https://doi.org/10.3390/biom12071002

AMA Style

Anitua E, Pino A, Azkargorta M, Elortza F, Prado R. High-Throughput Proteomic Analysis of Human Dermal Fibroblast Response to Different Blood Derivatives: Autologous Topical Serum Derived from Plasma Rich in Growth Factors (PRGF) versus Leukocyte- and Platelet-Rich Plasma (L-PRP). Biomolecules. 2022; 12(7):1002. https://doi.org/10.3390/biom12071002

Chicago/Turabian Style

Anitua, Eduardo, Ander Pino, Mikel Azkargorta, Felix Elortza, and Roberto Prado. 2022. "High-Throughput Proteomic Analysis of Human Dermal Fibroblast Response to Different Blood Derivatives: Autologous Topical Serum Derived from Plasma Rich in Growth Factors (PRGF) versus Leukocyte- and Platelet-Rich Plasma (L-PRP)" Biomolecules 12, no. 7: 1002. https://doi.org/10.3390/biom12071002

APA Style

Anitua, E., Pino, A., Azkargorta, M., Elortza, F., & Prado, R. (2022). High-Throughput Proteomic Analysis of Human Dermal Fibroblast Response to Different Blood Derivatives: Autologous Topical Serum Derived from Plasma Rich in Growth Factors (PRGF) versus Leukocyte- and Platelet-Rich Plasma (L-PRP). Biomolecules, 12(7), 1002. https://doi.org/10.3390/biom12071002

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