Drosophila melanogaster as a Model Organism for Obesity and Type-2 Diabetes Mellitus by Applying High-Sugar and High-Fat Diets
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fly Strains and Husbandry
2.2. Experimental Design
2.3. Survival
2.4. Climbing
2.5. Fly Weights
2.6. Glucose and Triglyceride Levels
2.7. Real-Time PCR Analysis
2.8. Statistical Analysis
3. Results
3.1. Survival
3.2. Weight Gain
3.3. Climbing
3.4. Glucose and Triglycerides
3.5. Drosophila Insulin-like Peptides
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ingredients | Control | High Sugar Diet (HSD) | High Fat Diet (HFD) |
---|---|---|---|
Water (mL) | 1075 | 1075 | 1075 |
Agar 1 (g) | 20 | 20 | 20 |
Sucrose 2 (g) | 100 | 300 | 100 |
Inactive yeast 3 (g) | 50 | 50 | 50 |
Pure coconut oil 4 (g) | 0 | 0 | 150 |
Tegosept 5 (20% in 95% ethanol) (mL) | 15 | 15 | 15 |
Propionic acid 6 | 3 | 3 | 3 |
Gene | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Accession No. (NM_...) |
---|---|---|---|
dIlp2 | AACGAGGTGCTGAGTATGGT | CGAACTCCTGGACAAACTGC | 079288 |
dIlp3 | ATCCTTATGATCGGCGGTGT | GTTCACGGGGTCCAAAGTTC | 140103 |
dIlp5 | TGATGGACATGCTGAGGGTT | CATGTGGTGAGATTCGGAGC | 206315 |
dIlp6 | TGGCGATGTATTTCCCAACAG | CCTTCACTATCCTTTGCAGTACT | 130644 |
rpl32 | GGCAAGCTTCAAGATGACCA | GTTCGATCCGTAACCGATGT | 170461 |
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Baenas, N.; Wagner, A.E. Drosophila melanogaster as a Model Organism for Obesity and Type-2 Diabetes Mellitus by Applying High-Sugar and High-Fat Diets. Biomolecules 2022, 12, 307. https://doi.org/10.3390/biom12020307
Baenas N, Wagner AE. Drosophila melanogaster as a Model Organism for Obesity and Type-2 Diabetes Mellitus by Applying High-Sugar and High-Fat Diets. Biomolecules. 2022; 12(2):307. https://doi.org/10.3390/biom12020307
Chicago/Turabian StyleBaenas, Nieves, and Anika E. Wagner. 2022. "Drosophila melanogaster as a Model Organism for Obesity and Type-2 Diabetes Mellitus by Applying High-Sugar and High-Fat Diets" Biomolecules 12, no. 2: 307. https://doi.org/10.3390/biom12020307