Concomitant Administration of Red Ginseng Extract with Lactic Acid Bacteria Increases the Plasma Concentration of Deglycosylated Ginsenosides in Healthy Human Subjects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. In Vitro RGE Fermentation Study with LAB
2.3. Pharmacokinetic Study
2.4. The Transport of Ginsenosides in Caco-2 and LLC-PK1-P-gp Cells
2.5. LC-MS/MS Analysis of Ginsenosides
2.6. Data Analysis
3. Results
3.1. Effect of LAB Supplementation on the In Vitro Ginsenoside Metabolism and In Vivo Pharmacokinetics of Ginsenosides in Human Subjects
3.2. LAB-Mediated Ginsenosides Metabolic Pathway
3.3. Permeability of Ginsenosides in CaCo-2 and LLC-PK1-P-gp Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ginsenosides | RGE | |||
---|---|---|---|---|
AUC (ng/mL·h) | Cmax (ng/mL) | Tmax (h) | MRT (h) | |
GRb1 | 184.6 ± 80.6 | 7.1 ± 3.2 | 5.3 ± 4.6 | 18.3 ± 1.1 |
GRb2 | 102.0 ± 51.0 | 3.9 ± 1.8 | 6.2 ± 4.0 | 18.5 ± 1.4 |
GRc | 118.4 ± 58.2 | 5.1 ± 2.4 | 6.2 ± 4.0 | 18.0 ± 1.2 |
GRd | 37.9 ± 12.6 | 2.1 ± 0.9 | 7.5 ± 5.8 | 18.2 ± 1.4 |
GRg3 | 94.1 ± 50.0 | 7.2 ± 4.8 | 7.2 ± 3.0 | 7.6 ± 2.8 |
GRh2 | 21.3 ± 10.1 | 2.1 ± 0.8 | 10.7 ± 0.6 | 17.0 ± 9.3 |
CK | 57.9 ± 16.6 | 5.1 ± 2.2 | 8.0 ± 4.0 | 17.2 ± 4.5 |
PPD | 129.5 ± 39.9 | 7.6 ± 1.7 | 16.0 ± 18.5 | 18.6 ± 5.0 |
PPT | 59.9 ± 49.2 | 9.5 ± 4.8 | 17.4 ± 17.4 | 19.4 ± 10.5 |
Ginsenosides | RGE + LAB | |||
AUC (ng/mL·h) | Cmax (ng/mL) | Tmax (h) | MRT (h) | |
GRb1 | 164.4 ± 77.3 | 6.2 ± 2.9 | 6.8 ± 1.1 | 16.9 ± 0.5 |
GRb2 | 88.6 ± 42.0 | 3.0 ± 1.4 | 7.2 ± 1.1 | 17.4 ± 0.1 |
GRc | 109.6 ± 50.3 | 4.6 ± 2.3 | 8.2 ± 1.8 | 16.8 ± 0.3 |
GRd | 24.1 ± 11.3 * | 0.9 ± 0.4 * | 7.6 ± 0.9 | 16.4 ± 0.8 |
GRg3 | 33.3 ± 21.7 * | 4.8 ± 3.1 | 6.4 ± 3.3 | 6.7 ± 0.7 |
GRh2 | 57.1 ± 22.0 * | 13.9 ± 12.8 | 10.6 ± 1.8 | 10.2 ± 1.6 |
CK | 123.3 ± 43.1 * | 12.6 ± 3.7 * | 9.6 ± 1.5 | 13.1 ± 2.6 |
PPD | 205.4 ± 81.7 * | 12.5 ± 4.1 * | 11.2 ± 1.6 | 16.6 ± 2.7 |
PPT | 211.2 ± 134.0 * | 18.0 ± 7.3 * | 10.6 ± 1.8 | 13.1 ± 4.5 |
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Jeon, J.-H.; Park, J.-H.; Jeon, S.Y.; Pang, M.; Choi, M.-K.; Song, I.-S. Concomitant Administration of Red Ginseng Extract with Lactic Acid Bacteria Increases the Plasma Concentration of Deglycosylated Ginsenosides in Healthy Human Subjects. Biomolecules 2022, 12, 1896. https://doi.org/10.3390/biom12121896
Jeon J-H, Park J-H, Jeon SY, Pang M, Choi M-K, Song I-S. Concomitant Administration of Red Ginseng Extract with Lactic Acid Bacteria Increases the Plasma Concentration of Deglycosylated Ginsenosides in Healthy Human Subjects. Biomolecules. 2022; 12(12):1896. https://doi.org/10.3390/biom12121896
Chicago/Turabian StyleJeon, Ji-Hyeon, Jin-Hyang Park, So Yeon Jeon, Minyeong Pang, Min-Koo Choi, and Im-Sook Song. 2022. "Concomitant Administration of Red Ginseng Extract with Lactic Acid Bacteria Increases the Plasma Concentration of Deglycosylated Ginsenosides in Healthy Human Subjects" Biomolecules 12, no. 12: 1896. https://doi.org/10.3390/biom12121896