Alteration of Cellular Energy Metabolism through LPAR2-Axin2 Axis in Gastric Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. LPA Treatment
2.4. Cell Proliferation Assay
2.5. ECIS Wound-Healing Assay (ECIS)
2.6. Luciferase Reporter Assay
2.7. Mitochondrial Bioenergetics
2.8. Data Analysis from the Clinical Cohort
2.9. Statistical Analysis
3. Results
3.1. Upregulation of LPA and LPAR2 in Gastric Cancer
3.2. LPA Stimulates Proliferation of Gastric Cancer Cells through LPAR2
3.3. LPA-Induced Motility of Gastric Cancer Cells through LPAR2 Receptor
3.4. LPA Mediates Gastric Cancer through LPAR2 and the β-Catenin Signaling Pathway
3.5. Silencing of LPAR2 by shRNA Inhibits LPA-Induced Progression of Gastric Cancer through the β-Catenin Signaling Pathway
3.6. LPA Treatment Stimulates Cellular Energy Metabolism through the LPAR2 Antagonist
3.7. Stabilization of Axin2 Rescued LPA-Induced Cellular Energy Metabolism and Progression of Gastric Cancer Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ara, H.; Subedi, U.; Sharma, P.; Bhattarai, S.; Sharma, S.; Manikandan, S.; Yu, X.; Bhuiyan, M.S.; Sun, H.; Miriyala, S.; et al. Alteration of Cellular Energy Metabolism through LPAR2-Axin2 Axis in Gastric Cancer. Biomolecules 2022, 12, 1805. https://doi.org/10.3390/biom12121805
Ara H, Subedi U, Sharma P, Bhattarai S, Sharma S, Manikandan S, Yu X, Bhuiyan MS, Sun H, Miriyala S, et al. Alteration of Cellular Energy Metabolism through LPAR2-Axin2 Axis in Gastric Cancer. Biomolecules. 2022; 12(12):1805. https://doi.org/10.3390/biom12121805
Chicago/Turabian StyleAra, Hosne, Utsab Subedi, Papori Sharma, Susmita Bhattarai, Sudha Sharma, Shrivats Manikandan, Xiuping Yu, Md. Shenuarin Bhuiyan, Hong Sun, Sumitra Miriyala, and et al. 2022. "Alteration of Cellular Energy Metabolism through LPAR2-Axin2 Axis in Gastric Cancer" Biomolecules 12, no. 12: 1805. https://doi.org/10.3390/biom12121805