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Article

Tropoelastin Promotes the Formation of Dense, Interconnected Endothelial Networks

1
Charles Perkins Centre, The University of Sydney, Camperdown, NSW 2006, Australia
2
School of Life and Environmental Sciences, The University of Sydney, Camperdown, NSW 2006, Australia
3
Sydney Nano Institute, The University of Sydney, Camperdown, NSW 2006, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Nuno M. Neves, Antonella Motta, Helena Ferreira and Rui L. Reis
Biomolecules 2021, 11(9), 1318; https://doi.org/10.3390/biom11091318
Received: 1 August 2021 / Revised: 30 August 2021 / Accepted: 2 September 2021 / Published: 6 September 2021
(This article belongs to the Special Issue Biological Biomaterials for Regenerative Medicine)
Tropoelastin, the soluble precursor of elastin, has been used for regenerative and wound healing purposes and noted for its ability to accelerate wound repair by enhancing vascularization at the site of implantation. However, it is not clear whether these effects are directly due to the interaction of tropoelastin with endothelial cells or communicated to endothelial cells following interactions between tropoelastin and neighboring cells, such as mesenchymal stem cells (MSCs). We adapted an endothelial tube formation assay to model in vivo vascularization with the goal of exploring the stimulatory mechanism of tropoelastin. In the presence of tropoelastin, endothelial cells formed less tubes, with reduced spreading into capillary-like networks. In contrast, conditioned media from MSCs that had been cultured on tropoelastin enhanced the formation of more dense, complex, and interconnected endothelial tube networks. This pro-angiogenic effect of tropoelastin is mediated indirectly through the action of tropoelastin on co-cultured cells. We conclude that tropoelastin inhibits endothelial tube formation, and that this effect is reversed by pro-angiogenic crosstalk from tropoelastin-treated MSCs. Furthermore, we find that the known in vivo pro-angiogenic effects of tropoelastin can be modeled in vitro, highlighting the value of tropoelastin as an indirect mediator of angiogenesis. View Full-Text
Keywords: tropoelastin; angiogenesis; endothelial tube formation assay; mesenchymal stem cells; endothelial cells tropoelastin; angiogenesis; endothelial tube formation assay; mesenchymal stem cells; endothelial cells
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MDPI and ACS Style

Al Halawani, A.; Abdulkhalek, L.; Mithieux, S.M.; Weiss, A.S. Tropoelastin Promotes the Formation of Dense, Interconnected Endothelial Networks. Biomolecules 2021, 11, 1318. https://doi.org/10.3390/biom11091318

AMA Style

Al Halawani A, Abdulkhalek L, Mithieux SM, Weiss AS. Tropoelastin Promotes the Formation of Dense, Interconnected Endothelial Networks. Biomolecules. 2021; 11(9):1318. https://doi.org/10.3390/biom11091318

Chicago/Turabian Style

Al Halawani, Aleen, Lea Abdulkhalek, Suzanne M. Mithieux, and Anthony S. Weiss 2021. "Tropoelastin Promotes the Formation of Dense, Interconnected Endothelial Networks" Biomolecules 11, no. 9: 1318. https://doi.org/10.3390/biom11091318

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