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Article

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning

Department for NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Benjamin Wylie
Biomolecules 2021, 11(5), 752; https://doi.org/10.3390/biom11050752
Received: 27 March 2021 / Revised: 9 May 2021 / Accepted: 11 May 2021 / Published: 18 May 2021
(This article belongs to the Special Issue Advances in Membrane Proteins 2021)
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively. View Full-Text
Keywords: magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field
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MDPI and ACS Style

Nimerovsky, E.; Movellan, K.T.; Zhang, X.C.; Forster, M.C.; Najbauer, E.; Xue, K.; Dervişoǧlu, R.; Giller, K.; Griesinger, C.; Becker, S.; Andreas, L.B. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning. Biomolecules 2021, 11, 752. https://doi.org/10.3390/biom11050752

AMA Style

Nimerovsky E, Movellan KT, Zhang XC, Forster MC, Najbauer E, Xue K, Dervişoǧlu R, Giller K, Griesinger C, Becker S, Andreas LB. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning. Biomolecules. 2021; 11(5):752. https://doi.org/10.3390/biom11050752

Chicago/Turabian Style

Nimerovsky, Evgeny, Kumar T. Movellan, Xizhou C. Zhang, Marcel C. Forster, Eszter Najbauer, Kai Xue, Rıza Dervişoǧlu, Karin Giller, Christian Griesinger, Stefan Becker, and Loren B. Andreas 2021. "Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning" Biomolecules 11, no. 5: 752. https://doi.org/10.3390/biom11050752

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