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Article

Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma

1
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal
2
Pharmacology and Pharmaceutical Care Laboratory, Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
3
Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
4
Instituto de Biofísica e Engenharia Biomédica, Faculdade de Ciências, Campo Grande, Universidade de Lisboa, 1749-016 Lisboa, Portugal
5
MED-Mediterranean Institute for Agriculture, Environment and Development, Department of Veterinary Medicine, University of Évora, Pólo da Mitra, 7002-554 Évora, Portugal
6
Centro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
7
CBIOS-Research Center for Biosciences & Health Technologies, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
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Faculty of Veterinary Medicine, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
9
Department of Biomedical Sciences, Faculty of Pharmacy, Campus Universitario, University of Alcalá, Ctra. A2 km 33,600, 28871 Alcalá de Henares, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors share senior authorship.
Academic Editor: Massimo Dal Monte
Biomolecules 2021, 11(4), 511; https://doi.org/10.3390/biom11040511
Received: 11 February 2021 / Revised: 25 March 2021 / Accepted: 25 March 2021 / Published: 30 March 2021
The global impact of cancer emphasizes the importance of developing innovative, effective and minimally invasive therapies. In the context of superficial cancers, the development of a multifunctional nanoparticle-based system and its in vitro and in vivo safety and efficacy characterization are, herein, proposed as a proof-of-concept. This multifunctional system consists of gold nanoparticles coated with hyaluronic and oleic acids, and functionalized with epidermal growth factor for greater specificity towards cutaneous melanoma cells. This nanoparticle system is activated by a near-infrared laser. The characterization of this nanoparticle system included several phases, with in vitro assays being firstly performed to assess the safety of gold nanoparticles without laser irradiation. Then, hairless immunocompromised mice were selected for a xenograft model upon inoculation of A375 human melanoma cells. Treatment with near-infrared laser irradiation for five minutes combined with in situ administration of the nanoparticles showed a tumor volume reduction of approximately 80% and, in some cases, led to the formation of several necrotic foci, observed histologically. No significant skin erythema at the irradiation zone was verified, nor other harmful effects on the excised organs. In conclusion, these assays suggest that this system is safe and shows promising results for the treatment of superficial melanoma. View Full-Text
Keywords: multifunctional nanoparticles; gold nanoparticles; targeted therapy; photothermal therapy; superficial tumors; melanoma; experimental models multifunctional nanoparticles; gold nanoparticles; targeted therapy; photothermal therapy; superficial tumors; melanoma; experimental models
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MDPI and ACS Style

Lopes, J.; Ferreira-Gonçalves, T.; Figueiredo, I.V.; Rodrigues, C.M.P.; Ferreira, H.; Ferreira, D.; Viana, A.S.; Faísca, P.; Gaspar, M.M.; Coelho, J.M.P.; Silva, C.O.; Reis, C.P. Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma. Biomolecules 2021, 11, 511. https://doi.org/10.3390/biom11040511

AMA Style

Lopes J, Ferreira-Gonçalves T, Figueiredo IV, Rodrigues CMP, Ferreira H, Ferreira D, Viana AS, Faísca P, Gaspar MM, Coelho JMP, Silva CO, Reis CP. Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma. Biomolecules. 2021; 11(4):511. https://doi.org/10.3390/biom11040511

Chicago/Turabian Style

Lopes, Joana, Tânia Ferreira-Gonçalves, Isabel V. Figueiredo, Cecília M.P. Rodrigues, Hugo Ferreira, David Ferreira, Ana S. Viana, Pedro Faísca, Maria M. Gaspar, João M.P. Coelho, Catarina O. Silva, and Catarina P. Reis 2021. "Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma" Biomolecules 11, no. 4: 511. https://doi.org/10.3390/biom11040511

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