Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals
2.3. Experimental Design
2.4. Tissue Homogenization and Protein Quantification
2.5. Western Blotting
2.6. Phosphorylation of Insulin Receptor
2.7. Multiplexed Bead Immunoassays
2.8. Statistical Analysis
3. Results
3.1. General Characteristics of the Experimental Groups
3.2. Leptin Modifies the Activation of Inflammatory Targets and Cytokine Levels in Inguinal Fat and Liver in an Opposed Manner
3.3. Inguinal IRS1/PI3K Signaling Is Reduced in Leptin-Treated Rats
3.4. Chronic Leptin Exposure Increases Hepatic IRS1/PI3K Signaling
3.5. Changes in Akt Activation Are Inversely Related to STAT3 and JNK Phosphorylation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Control | Pair-Fed | Leptin |
---|---|---|---|
Inguinal fat weight (%) | 1.02 ± 0.08 | 0.94 ± 0.09 | 0.64 ± 0.08 ** ## |
Liver weight (%) | 3.62 ± 0.23 | 3.82 ± 0.13 | 3.99 ± 0.10 |
Serum IL-1β (pg/mL) | 25.56 ± 2.63 | 17.63 ± 2.40 | 39.32 ± 4.77 * # |
Serum IL-17A (pg/mL) | 8.95 ± 1.55 | 7.51 ± 1.02 | 6.40 ± 0.78 |
Serum MCP-1 (pg/mL) | 36.96 ± 3.82 | 31.48 ± 2.87 | 36.86 ± 3.67 |
Serum IL-4 (pg/mL) | 3.25 ± 0.31 | 2.93 ± 0.60 | 11.09 ± 1.00 *** ### |
Serum IL-13 (pg/mL) | 1.95 ± 0.18 | 3.13 ± 0.30 * | 5.65 ± 0.62 * # |
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Barrios, V.; Campillo-Calatayud, A.; Guerra-Cantera, S.; Canelles, S.; Martín-Rivada, Á.; Frago, L.M.; Chowen, J.A.; Argente, J. Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment. Biomolecules 2021, 11, 1734. https://doi.org/10.3390/biom11111734
Barrios V, Campillo-Calatayud A, Guerra-Cantera S, Canelles S, Martín-Rivada Á, Frago LM, Chowen JA, Argente J. Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment. Biomolecules. 2021; 11(11):1734. https://doi.org/10.3390/biom11111734
Chicago/Turabian StyleBarrios, Vicente, Ana Campillo-Calatayud, Santiago Guerra-Cantera, Sandra Canelles, Álvaro Martín-Rivada, Laura M. Frago, Julie A. Chowen, and Jesús Argente. 2021. "Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment" Biomolecules 11, no. 11: 1734. https://doi.org/10.3390/biom11111734