Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Overproduction and Purification of HU Protein
2.2. Tritium Labeling of HU Protein
2.3. Topoisomers of Plasmid pRLM4
2.4. Gel Retardation Assays
2.5. Topological Assays
2.6. Nick Closure Assay
2.7. Chemical Crosslinking
3. Results
3.1. Binding of HU to Negatively Supercoiled and Nicked Circular DNA
3.2. Affinities of HU for Different Forms of pRLM4
3.3. Binding of HU to pRLM4 Topoisomers
3.4. Constraining of Negative Supercoils by HU
3.5. Chemical Crosslinking of DNA-Bound HU
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, L.; Zhang, Z.; McMacken, R. Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA. Biomolecules 2021, 11, 1724. https://doi.org/10.3390/biom11111724
Huang L, Zhang Z, McMacken R. Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA. Biomolecules. 2021; 11(11):1724. https://doi.org/10.3390/biom11111724
Chicago/Turabian StyleHuang, Li, Zhenfeng Zhang, and Roger McMacken. 2021. "Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA" Biomolecules 11, no. 11: 1724. https://doi.org/10.3390/biom11111724