Next Article in Journal
Ranking Series of Cancer-Related Gene Expression Data by Means of the Superposing Significant Interaction Rules Method
Previous Article in Journal
Direct Observation of Sophorolipid Micelle Docking in Model Membranes and Cells by Single Particle Studies Reveals Optimal Fusion Conditions
Open AccessArticle

Eicosapentaenoic Acid Regulates Inflammatory Pathways through Modulation of Transcripts and miRNA in Adipose Tissue of Obese Mice

1
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
2
Department of Nutritional Sciences, Texas Tech University, Lubbock, TX 79409, USA
3
Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USA
4
Department of Physiology, Faculty of Medicine, University of Peradeniya, Peradeniya 20400, Sri Lanka
*
Authors to whom correspondence should be addressed.
Current affiliation: Department of Nutrition and Hospitality Management, University of Mississippi, MS 38677, USA.
Current affiliation: Department of Nutrition and Food Studies, Syracuse University, Syracuse, NY 13210, USA.
Biomolecules 2020, 10(9), 1292; https://doi.org/10.3390/biom10091292
Received: 7 July 2020 / Revised: 31 August 2020 / Accepted: 1 September 2020 / Published: 7 September 2020
This study aims to investigate the global profiling of genes and miRNAs expression to explore the regulatory effects of eicosapentaenoic acid (EPA) in visceral adipose tissue (VAT) of obese mice. We used male mice, fed either a high-fat diet (HF) or HF supplemented with EPA (HF-EPA), for 11 weeks. RNA, and small RNA profiling, were performed by RNAseq analysis. We conducted analyses using Ingenuity Pathway Analysis software (IPA®) and validated candidate genes and miRNAs related to lipid mediators and inflammatory pathways using qRT-PCR. We identified 153 genes differentially downregulated, and 62 microRNAs differentially expressed in VAT from HF-EPA compared to HF. Genes with a positive association with inflammation, chemotaxis, insulin resistance, and inflammatory cell death, such as Irf5, Alox5ap, Tlrs, Cd84, Ccr5, Ccl9, and Casp1, were downregulated by EPA. Moreover, EPA significantly reduced LTB4 levels, a lipid mediator with a central role in inflammation and insulin resistance in obesity. The pathways and mRNA/microRNA interactions identified in our study corroborated with data validated for inflammatory genes and miRNAs. Together, our results identified key VAT inflammatory targets and pathways, which are regulated by EPA. These targets merit further investigation to better understand the protective mechanisms of EPA in obesity-associated inflammation. View Full-Text
Keywords: obesity; adipose tissue; inflammation; leukotriene-B4; eicosapentaenoic acid obesity; adipose tissue; inflammation; leukotriene-B4; eicosapentaenoic acid
Show Figures

Figure 1

MDPI and ACS Style

Ramalho, T.; Pahlavani, M.; Kalupahana, N.; Wijayatunga, N.; Ramalingam, L.; Jancar, S.; Moustaid-Moussa, N. Eicosapentaenoic Acid Regulates Inflammatory Pathways through Modulation of Transcripts and miRNA in Adipose Tissue of Obese Mice. Biomolecules 2020, 10, 1292.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop