Next Article in Journal
Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs
Next Article in Special Issue
IL-17A as a Potential Therapeutic Target for Patients on Peritoneal Dialysis
Previous Article in Journal
Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson’s Disease Patients and Is Present within Lewy Bodies
Previous Article in Special Issue
Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis
Article

Alanyl-Glutamine Restores Tight Junction Organization after Disruption by a Conventional Peritoneal Dialysis Fluid

1
Division of Pediatric Nephrology, Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany
2
Christian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria
3
Division of Pediatric Nephrology and Gastroenterology, Department of Pediatrics and Adolescent Medicine, Comprehensive Center for Pediatrics, Medical University of Vienna, 1090 Vienna, Austria
4
Kirchhoff Institute for Physics, Heidelberg University, 69120 Heidelberg, Germany
5
MTA-SE, Pediatrics and Nephrology Research Group, 1083 Budapest, Hungary
6
1st Department of Pediatrics, Semmelweis University, 1083 Budapest, Hungary
7
Immunology and Cellular Biology Department, Molecular Biology Centre Severo Ochoa, 28049 Madrid, Spain
8
General, Visceral and Transplantation Surgery, Heidelberg University, 69120 Heidelberg, Germany
9
German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
10
Department of Physiology, Faculty of Medicine, University of Thessaly, 41500 Larissa, Greece
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(8), 1178; https://doi.org/10.3390/biom10081178
Received: 16 July 2020 / Revised: 7 August 2020 / Accepted: 11 August 2020 / Published: 13 August 2020
(This article belongs to the Special Issue Molecular Mechanisms of Peritoneal Membrane Pathophysiology)
Understanding and targeting the molecular basis of peritoneal solute and protein transport is essential to improve peritoneal dialysis (PD) efficacy and patient outcome. Supplementation of PD fluids (PDF) with alanyl-glutamine (AlaGln) increased small solute transport and reduced peritoneal protein loss in a recent clinical trial. Transepithelial resistance and 10 kDa and 70 kDa dextran transport were measured in primary human endothelial cells (HUVEC) exposed to conventional acidic, glucose degradation products (GDP) containing PDF (CPDF) and to low GDP containing PDF (LPDF) with and without AlaGln. Zonula occludens-1 (ZO-1) and claudin-5 were quantified by Western blot and immunofluorescence and in mice exposed to saline and CPDF for 7 weeks by digital imaging analyses. Spatial clustering of ZO-1 molecules was assessed by single molecule localization microscopy. AlaGln increased transepithelial resistance, and in CPDF exposed HUVEC decreased dextran transport rates and preserved claudin-5 and ZO-1 abundance. Endothelial clustering of membrane bound ZO-1 was higher in CPDF supplemented with AlaGln. In mice, arteriolar endothelial claudin-5 was reduced in CPDF, but restored with AlaGln, while mesothelial claudin-5 abundance was unchanged. AlaGln supplementation seals the peritoneal endothelial barrier, and when supplemented to conventional PD fluid increases claudin-5 and ZO-1 abundance and clustering of ZO-1 in the endothelial cell membrane. View Full-Text
Keywords: peritoneal dialysis; tight junctions; paracellular transport; alanyl-glutamine peritoneal dialysis; tight junctions; paracellular transport; alanyl-glutamine
Show Figures

Figure 1

MDPI and ACS Style

Bartosova, M.; Herzog, R.; Ridinger, D.; Levai, E.; Jenei, H.; Zhang, C.; González Mateo, G.T.; Marinovic, I.; Hackert, T.; Bestvater, F.; Hausmann, M.; López Cabrera, M.; Kratochwill, K.; Zarogiannis, S.G.; Schmitt, C.P. Alanyl-Glutamine Restores Tight Junction Organization after Disruption by a Conventional Peritoneal Dialysis Fluid. Biomolecules 2020, 10, 1178. https://doi.org/10.3390/biom10081178

AMA Style

Bartosova M, Herzog R, Ridinger D, Levai E, Jenei H, Zhang C, González Mateo GT, Marinovic I, Hackert T, Bestvater F, Hausmann M, López Cabrera M, Kratochwill K, Zarogiannis SG, Schmitt CP. Alanyl-Glutamine Restores Tight Junction Organization after Disruption by a Conventional Peritoneal Dialysis Fluid. Biomolecules. 2020; 10(8):1178. https://doi.org/10.3390/biom10081178

Chicago/Turabian Style

Bartosova, Maria, Rebecca Herzog, David Ridinger, Eszter Levai, Hanna Jenei, Conghui Zhang, Guadalupe T. González Mateo, Iva Marinovic, Thilo Hackert, Felix Bestvater, Michael Hausmann, Manuel López Cabrera, Klaus Kratochwill, Sotirios G. Zarogiannis, and Claus P. Schmitt 2020. "Alanyl-Glutamine Restores Tight Junction Organization after Disruption by a Conventional Peritoneal Dialysis Fluid" Biomolecules 10, no. 8: 1178. https://doi.org/10.3390/biom10081178

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop