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Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing 100193, China
2
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 986; https://doi.org/10.3390/biom10070986
Received: 5 May 2020 / Revised: 24 June 2020 / Accepted: 26 June 2020 / Published: 1 July 2020
(This article belongs to the Section Biomacromolecules)
Fluoroacetamide (FAM) is a small (77 Da) and highly toxic chemical, formerly used as a rodenticide and potentially as a poison by terrorists. Poisoning with FAM has occurred in humans, but few reliably rapid detection methods and antidotes have been reported. Therefore, producing a specific antibody to FAM is not only critical for the development of a fast diagnostic but also a potential treatment. However, achieving this goal is a great challenge, mainly due to the very low molecular weight of FAM. Here, we design two groups of FAM haptens for the first time, maximally exposing the fluorine or amino groups, with the aid of linear aliphatic or phenyl-contained spacer arms. Interestingly, whereas the hapten with fluorine at the far end of the hapten did not induce an antibody response to FAM, the hapten with an amino group at the far end and phenyl-contained spacer arm triggered a significantly specific antibody response. Finally, a monoclonal antibody (mAb) named 5D11 was successfully obtained with an IC50 value of 97 μg mL−1 and negligible cross-reactivities to the other nine functional and structural analogs. View Full-Text
Keywords: fluoroacetamide; hapten design and synthesis; antibodies; toxins; immunoassay fluoroacetamide; hapten design and synthesis; antibodies; toxins; immunoassay
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MDPI and ACS Style

Yang, L.; Zhang, X.; Shen, D.; Yu, X.; Li, Y.; Wen, K.; Shen, J.; Wang, Z. Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical. Biomolecules 2020, 10, 986.

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