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Open AccessArticle

Evaluation of Resin-Based Material Containing Copaiba Oleoresin (Copaifera Reticulata Ducke): Biological Effects on the Human Dental Pulp Stem Cells

1
Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, São Paulo, SP 05508-060, Brazil
2
School of Dentistry, Federal University of Pará, Belém, PA 66075-110, Brazil
3
Postgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University, São Paulo, SP 02112-000, Brazil
4
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizionte, MG 31270-901, Brazil
5
Departamento de Ciências da Vida, Federal University of Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil
6
Institute of Biological Sciences, Federal University of Pará, Belém, PA 66075-110, Brazil
7
Post graduation course in Dentistry, Ibirapuera University, São Paulo, SP 04661-100, Brazil
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 972; https://doi.org/10.3390/biom10070972
Received: 9 June 2020 / Revised: 20 June 2020 / Accepted: 23 June 2020 / Published: 28 June 2020
(This article belongs to the Special Issue Application and Mechanism of Natural Compounds in Dentistry)
The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2. View Full-Text
Keywords: stem cells; dental pulp; MTA; pulp capping; pulp repair stem cells; dental pulp; MTA; pulp capping; pulp repair
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Couto, R.S.D.; Rodrigues, M.F.S.D.; Ferreira, L.S.; Diniz, I.M.A.; Silva, F.S.; Lopez, T.C.C.; Lima, R.R.; Marques, M.M. Evaluation of Resin-Based Material Containing Copaiba Oleoresin (Copaifera Reticulata Ducke): Biological Effects on the Human Dental Pulp Stem Cells. Biomolecules 2020, 10, 972.

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