Next Article in Journal
Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation
Previous Article in Journal
Phytochemical and Biological Screening of Oenothera biennis L. Hydroalcoholic Extract
Article

An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody

1
Medicinal Bioconvergence Research Center, College of Pharmacy and College of Medicine, Gangnam Severance Hospital, Yonsei University, Incheon 21983, Korea
2
Department of Life Science, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea
3
Department of Internal Medicine, Yonsei University College of Medicine, 63 Gil-20, Eonju-ro, Gangnam-gu, Seoul 06229, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2020, 10(6), 820; https://doi.org/10.3390/biom10060820
Received: 8 May 2020 / Revised: 22 May 2020 / Accepted: 25 May 2020 / Published: 27 May 2020
(This article belongs to the Section Cellular Biochemistry)
AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established; however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes. View Full-Text
Keywords: antibody; splice variant; AIMP2-DX2; phage display; diagnostic marker antibody; splice variant; AIMP2-DX2; phage display; diagnostic marker
Show Figures

Graphical abstract

MDPI and ACS Style

Kim, D.G.; Nguyen, T.T.H.; Kwon, N.H.; Sung, J.; Lim, S.; Kang, E.-J.; Lee, J.; Seo, W.Y.; Kim, A.; Chang, Y.S.; Shim, H.; Kim, S. An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody. Biomolecules 2020, 10, 820. https://doi.org/10.3390/biom10060820

AMA Style

Kim DG, Nguyen TTH, Kwon NH, Sung J, Lim S, Kang E-J, Lee J, Seo WY, Kim A, Chang YS, Shim H, Kim S. An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody. Biomolecules. 2020; 10(6):820. https://doi.org/10.3390/biom10060820

Chicago/Turabian Style

Kim, Dae G., Thi T.H. Nguyen, Nam H. Kwon, Junsik Sung, Semi Lim, Eun-Joo Kang, Jihye Lee, Woo Y. Seo, Arum Kim, Yoon S. Chang, Hyunbo Shim, and Sunghoon Kim. 2020. "An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody" Biomolecules 10, no. 6: 820. https://doi.org/10.3390/biom10060820

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop