Next Article in Journal
Large-Scale Differential Gene Expression Transcriptomic Analysis Identifies a Metabolic Signature Shared by All Cancer Cells
Next Article in Special Issue
Excretory/Secretory Metabolome of the Zoonotic Roundworm Parasite Toxocara canis
Previous Article in Journal
The Antitumor Agent Ansamitocin P-3 Binds to Cell Division Protein FtsZ in Actinosynnema pretiosum
Previous Article in Special Issue
Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma
Open AccessArticle

The Genoprotective Role of Naringin

1
Department of Gastronomy Sciences and Functional Foods, Poznań University of Life Sciences, 60-637 Poznań, Poland
2
Department of Industrial Products Quality and Packaging Development, Poznań University of Economics and Business, 61-875 Poznań, Poland
3
Department of Agronomy, Poznań University of Life Sciences, 60-637 Poznań, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(5), 700; https://doi.org/10.3390/biom10050700
Received: 15 February 2020 / Revised: 26 April 2020 / Accepted: 27 April 2020 / Published: 30 April 2020
Since ancient times, fruits and edible plants have played a special role in the human diet for enhancing health and maintaining youthfulness. The aim of our work was to determine the interactions between naringin, a natural ingredient of grapefruits, and DNA using an electrochemical biosensor. Electrochemical methods allow analyzing the damages occurring in the structure of nucleic acids and their interactions with xenobiotics. Our study showed that the changes in the location of electrochemical signals and their intensity resulted from the structural alterations in DNA. The signal of adenine was affected at lower concentrations of naringin, but the signal of guanine was unaffected in the same condition. The dynamics of changes occurring in the peak height and surface of adenine related to naringin concentration was also significantly lower. The complete binding of all adenine bases present in the tested double-stranded DNA solution was observed at naringin concentrations ranging from 8.5 to 10.0 µM. At larger concentrations, this active compound exerted an oxidizing effect on DNA. However, the critical concentrations of naringin were found to be more than twice as high as the dose absorbable in an average human (4 µM). The results of our work might be helpful in the construction of electrochemical sensors for testing the content of polyphenols and would allow determining their genoprotective functionality. View Full-Text
Keywords: naringin; DNA; reactive oxygen species; electrochemical DNA biosensor; square-wave voltammetry naringin; DNA; reactive oxygen species; electrochemical DNA biosensor; square-wave voltammetry
Show Figures

Figure 1

MDPI and ACS Style

Szczepaniak, O.; Ligaj, M.; Kobus-Cisowska, J.; Tichoniuk, M.; Dziedziński, M.; Przeor, M.; Szulc, P. The Genoprotective Role of Naringin. Biomolecules 2020, 10, 700.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop