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Therapeutic Efficacy of Nyctanthes arbor-tristis Flowers to Inhibit Proliferation of Acute and Chronic Primary Human Leukemia Cells, with Adipocyte Differentiation and in Silico Analysis of Interactions between Survivin Protein and Selected Secondary Metabolites

1
Department of Biomedical Sciences, British College of Applied Studies, BCAS City Campus, Colombo 00600, Sri Lanka
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Department of Zoology, Faculty of Applied Sciences (Center for Biotechnology), University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka
3
Medical Research Core Facility and Platforms, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Research, Riyadh 11481, Kingdom of Saudi Arabia
4
Department of Chemistry, Faculty of Applied Sciences (Center for Instrumentation Facility), University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 165; https://doi.org/10.3390/biom10020165
Received: 22 October 2019 / Revised: 19 December 2019 / Accepted: 6 January 2020 / Published: 21 January 2020
Although the antidiabetic efficacy of Nyctanthes arbor-tristis flowers has been reported, antiproliferative and anti-obesity activities are yet to be explored. We examined the anti-obesity and antiproliferative potentials of different fractions (hexane, chloroform, ethyl acetate, methanol) of N. abor-tristis flower extract for the first time using 3T3-L1 cells, primary peripheral blood mononuclear cells (PBMC) isolated from healthy and adult acute myeloid (AML) and chronic lymphocytic leukemia (CLL) patients, recombinant Jurkat T cells, and MCF7 cell lines. The in vitro hypoglycemic activity was evaluated using the inhibition of α-amylase enzyme and glucose uptake by yeast cells. The percentage glucose uptake and α-amylase inhibitory activity increased in a dose-dependent manner in the crude and the tested fractions (hexane and ethyl acetate). Inhibition of the 3T3-L1 cells’ differentiation was observed in the ethyl acetate and chloroform fractions, followed by the hexane fraction. Antiproliferative analyses revealed that Nyctanthes exerted a high specific activity against anti-AML and anti-CLL PBMC cells, especially by the hexane and ethyl acetate fractions. The gas chromatography/mass spectrometry analysis indicated the presence of 1-heptacosanol (hexane fraction), 1-octadecene (hexane and chloroform fractions), and other organic compounds. Molecular docking demonstrated that phenol,2,5-bis(1,1-dimethylethyl) and 4-hydroxypyridine 1-oxide compounds showed specificity toward survivin protein, indicating the feasibility of N. abor-tristis in developing new drug leads against leukemia. View Full-Text
Keywords: Nyctanthes abor-tristis; human breast carcinoma; primary peripheral blood mononuclear cells; acute myeloid leukemia; chronic lymphocytic leukemia; obesity; hypoglycemia; molecular docking; survivin protein; gas chromatography/mass spectrometry Nyctanthes abor-tristis; human breast carcinoma; primary peripheral blood mononuclear cells; acute myeloid leukemia; chronic lymphocytic leukemia; obesity; hypoglycemia; molecular docking; survivin protein; gas chromatography/mass spectrometry
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Heendeniya, S.N.; Keerthirathna, L.R.; Manawadu, C.K.; Dissanayake, I.H.; Ali, R.; Mashhour, A.; Alzahrani, H.; Godakumbura, P.; Boudjelal, M.; Peiris, D.C. Therapeutic Efficacy of Nyctanthes arbor-tristis Flowers to Inhibit Proliferation of Acute and Chronic Primary Human Leukemia Cells, with Adipocyte Differentiation and in Silico Analysis of Interactions between Survivin Protein and Selected Secondary Metabolites. Biomolecules 2020, 10, 165.

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