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Open AccessArticle

Separation and Purification of Biogenic 1,3-Propanediol from Fermented Glycerol through Flocculation and Strong Acidic Ion-Exchange Resin

1
Institute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3–5, 400372 Cluj-Napoca, Romania
2
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3–5, 400372 Cluj-Napoca, Romania
3
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3–5, 400372 Cluj-Napoca, Romania
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(12), 1601; https://doi.org/10.3390/biom10121601
Received: 5 October 2020 / Revised: 13 November 2020 / Accepted: 25 November 2020 / Published: 26 November 2020
In the present work, was investigated the separation and purification procedure of the biogenic 1,3-propanediol (1,3-PD), which is a well-known valuable compound in terms of bio-based plastic materials development. The biogenic 1,3-PD was obtained as a major metabolite through the glycerol fermentation by Klebsiella pneumoniae DSMZ 2026 and was subjected to separation and purification processes. A strong acidic ion exchange resin in H+ form was used for 1,3-PD purification from the aqueous solution previously obtained by broth flocculation. The eluent volume was investigated considering the removal of the secondary metabolites such as organic acids (acetic, citric, lactic, and succinic acids) and 2,3-butanediol (2,3-BD), and unconsumed glycerol. It was observed that a volume of 84 mL of ethanol 75% loaded with a flow rate of 7 mL/min completely remove the secondary metabolites from 10 mL of concentrated fermented broth, and pure biogenic 1,3-PD was recovered in 128 mL of the eluent. View Full-Text
Keywords: 1,3-propanediol; cationic resin; flocculation; Klebsiella pneumoniae; purification 1,3-propanediol; cationic resin; flocculation; Klebsiella pneumoniae; purification
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MDPI and ACS Style

Mitrea, L.; Leopold, L.F.; Bouari, C.; Vodnar, D.C. Separation and Purification of Biogenic 1,3-Propanediol from Fermented Glycerol through Flocculation and Strong Acidic Ion-Exchange Resin. Biomolecules 2020, 10, 1601. https://doi.org/10.3390/biom10121601

AMA Style

Mitrea L, Leopold LF, Bouari C, Vodnar DC. Separation and Purification of Biogenic 1,3-Propanediol from Fermented Glycerol through Flocculation and Strong Acidic Ion-Exchange Resin. Biomolecules. 2020; 10(12):1601. https://doi.org/10.3390/biom10121601

Chicago/Turabian Style

Mitrea, Laura; Leopold, Loredana F.; Bouari, Cosmina; Vodnar, Dan C. 2020. "Separation and Purification of Biogenic 1,3-Propanediol from Fermented Glycerol through Flocculation and Strong Acidic Ion-Exchange Resin" Biomolecules 10, no. 12: 1601. https://doi.org/10.3390/biom10121601

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