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Article

Preventive Effect of Muscone against Cisplatin Nephrotoxicity in LLC-PK1 Cells

by 1,†, 2,†, 3,* and 1,*
1
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Korea
3
Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2020, 10(10), 1444; https://doi.org/10.3390/biom10101444
Received: 1 September 2020 / Revised: 6 October 2020 / Accepted: 12 October 2020 / Published: 15 October 2020
Cisplatin, one of the most common antitumor agents, is widely applied to treat various cancerous diseases and is included in the World Health Organization Model List of Essential Medicines. Cisplatin therapy is used to treat 10–20% of all cancerous cases, and its cure rate is especially high in testicular cancer (over 90%). However, a major side effect of this anticancer drug is nephrotoxicity, limiting treatment effect and reducing the quality of life in cancer patients. Muscone, an odoriferous constituent of musk, was confirmed to inhibit cisplatin-induced LLC-PK1 kidney proximal tubule cell death in a dose-dependent manner. In term of renal protective mechanism, muscone inhibited cisplatin oxidative toxicity by decreasing reactive oxygen species (ROS) level and stimulating HO-1 expression. Muscone also exerted anti-inflammation effect through inhibition of p38 phosphorylation. Furthermore, muscone mitigated cisplatin-induced apoptosis in LLC-PK1 cells via both intrinsic and extrinsic pathways by inhibiting pro-apoptotic protein Bax expression, and cleaved caspase-3, 7, and 8; and increase of anti-apoptotic protein Bcl-2 level. In addition, the anti-apoptotic effect of muscone also was enhanced by preventing p53 expression and its phosphorylation. Our study showed that muscone may be a potential protective agent against cisplatin-induced nephrotoxicity. View Full-Text
Keywords: muscone; cisplatin; nephrotoxicity; acute kidney injury; LLC-PK1; chromatin condensation; apoptosis; oxidative stress; inflammation; p53 muscone; cisplatin; nephrotoxicity; acute kidney injury; LLC-PK1; chromatin condensation; apoptosis; oxidative stress; inflammation; p53
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MDPI and ACS Style

Phung, H.M.; Lee, S.; Hwang, J.H.; Kang, K.S. Preventive Effect of Muscone against Cisplatin Nephrotoxicity in LLC-PK1 Cells. Biomolecules 2020, 10, 1444. https://doi.org/10.3390/biom10101444

AMA Style

Phung HM, Lee S, Hwang JH, Kang KS. Preventive Effect of Muscone against Cisplatin Nephrotoxicity in LLC-PK1 Cells. Biomolecules. 2020; 10(10):1444. https://doi.org/10.3390/biom10101444

Chicago/Turabian Style

Phung, Hung M., Sullim Lee, Ji H. Hwang, and Ki S. Kang 2020. "Preventive Effect of Muscone against Cisplatin Nephrotoxicity in LLC-PK1 Cells" Biomolecules 10, no. 10: 1444. https://doi.org/10.3390/biom10101444

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