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Open AccessArticle

Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses

1
Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 63243, Korea
2
Department of Ocean Sciences, Memorial University of Newfoundland, St. John’s, NL A1C 5S7, Canada
3
Sri Lanka Institute of Nanotechnology (SLINTEC), Nanotechnology and Science Park, Mahenwatta, Pitipana, Homagama 10206, Sri Lanka
4
Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka
5
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 1X5, Canada
6
Marine Science Institute, Jeju National University, Jeju Self-Governing Province 63333, Korea
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(10), 1382; https://doi.org/10.3390/biom10101382
Received: 12 July 2020 / Revised: 8 August 2020 / Accepted: 22 September 2020 / Published: 28 September 2020
CXCL8 (interleukin-8, IL-8) is a CXC family chemokine that recruits specific target cells and mediates inflammation and wound healing. This study reports the identification and characterization of two cxcl8 homologs from rock bream, Oplegnathus fasciatus. Investigation of molecular signature, homology, phylogeny, and gene structure suggested that they belonged to lineages 1 (L1) and 3 (L3), and designated Ofcxcl8-L1 and Ofcxcl8-L3. While Ofcxcl8-L1 and Ofcxcl8-L3 revealed quadripartite and tripartite organization, in place of the mammalian ELR (Glu-Leu-Arg) motif, their peptides harbored EMH (Glu-Met-His) and NSH (Asn-Ser-His) motifs, respectively. Transcripts of Ofcxcl8s were constitutively detected by Quantitative Real-Time PCR (qPCR) in 11 tissues examined, however, at different levels. Ofcxcl8-L1 transcript robustly responded to treatments with stimulants, such as flagellin, concanavalin A, lipopolysaccharide, and poly(I:C), and pathogens, including Edwardsiella tarda, Streptococcus iniae, and rock bream iridovirus, when compared with Ofcxcl8-L3 mRNA. The differences in the putative promoter features may partly explain the differential transcriptional modulation of Ofcxcl8s. Purified recombinant OfCXCL8 (rOfCXCL8) proteins were used in in vitro chemotaxis and proliferation assays. Despite the lack of ELR motif, both rOfCXCL8s exhibited leukocyte chemotactic and proliferative functions, where the potency of rOfCXCL8-L1 was robust and significant compared to that of rOfCXCL8-L3. The results, taken together, are indicative of the crucial importance of Ofcxcl8s in inflammatory responses and immunoregulatory roles in rock bream immunity. View Full-Text
Keywords: CXC chemokine; CXCL8; interleukin-8 (il-8); molecular profiling; genomic sequence arrangement; promoter prediction analyses; transcriptional expression; chemotaxis; leukocyte proliferation CXC chemokine; CXCL8; interleukin-8 (il-8); molecular profiling; genomic sequence arrangement; promoter prediction analyses; transcriptional expression; chemotaxis; leukocyte proliferation
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MDPI and ACS Style

Umasuthan, N.; Bathige, S.; Thulasitha, W.S.; Oh, M.; Lee, J. Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses. Biomolecules 2020, 10, 1382. https://doi.org/10.3390/biom10101382

AMA Style

Umasuthan N, Bathige S, Thulasitha WS, Oh M, Lee J. Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses. Biomolecules. 2020; 10(10):1382. https://doi.org/10.3390/biom10101382

Chicago/Turabian Style

Umasuthan, Navaneethaiyer; Bathige, SDNK; Thulasitha, William S.; Oh, Minyoung; Lee, Jehee. 2020. "Comparative Characterization of Two cxcl8 Homologs in Oplegnathus fasciatus: Genomic, Transcriptional and Functional Analyses" Biomolecules 10, no. 10: 1382. https://doi.org/10.3390/biom10101382

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