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Open AccessArticle

Untargeted Metabolomics Approach Reveals Diverse Responses of Pastinaca Sativa to Ozone and Wounding Stresses

1
INRA, LAE, Université de Lorraine, 54000 Nancy France
2
Université de Lorraine, AgroParisTech, INRA, UMR Silva, 54000 Nancy, France
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(7), 153; https://doi.org/10.3390/metabo9070153
Received: 6 May 2019 / Revised: 8 July 2019 / Accepted: 20 July 2019 / Published: 23 July 2019
(This article belongs to the Special Issue Metabolomic and Flux Analysis in Plants)
Stresses such as wounding or atmospheric pollutant exposure have a significant impact on plant fitness. Since it has been widely described that the metabolome directly reflects plant physiological status, a way to assess this impact is to perform a global metabolomic analysis. In this study, we investigated the effect of two abiotic stresses (mechanical wounding and ozone exposure) on parsnip metabolic balance using a liquid chromatography-mass spectrometry-based untargeted metabolomic approach. For this purpose, parsnip leaves were submitted to an acute ozone exposure or were mechanically wounded and sampled 24, 48, and 72 h post-treatment. Multivariate and univariate statistical analyses highlighted numerous differentially-accumulated metabolic features as a function of time and treatment. Mechanical wounding led to a more differentiated response than ozone exposure. We found that the levels of coumarins and fatty acyls increased in wounded leaves, while flavonoid concentration decreased in the same conditions. These results provide an overview of metabolic destabilization through differentially-accumulated compounds and provide a better understanding of global plant metabolic changes in defense mechanisms. View Full-Text
Keywords: metabolomics; elicitation; parsnip; wounding; ozone metabolomics; elicitation; parsnip; wounding; ozone
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MDPI and ACS Style

Galati, G.; Gandin, A.; Jolivet, Y.; Larbat, R.; Hehn, A. Untargeted Metabolomics Approach Reveals Diverse Responses of Pastinaca Sativa to Ozone and Wounding Stresses. Metabolites 2019, 9, 153.

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