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Metabolites 2019, 9(4), 68; https://doi.org/10.3390/metabo9040068

Drying Enhances Signal Intensities for Global GC–MS Metabolomics

Max-Planck Institute for Marine Microbiology, Celsiusstr. 1, 28359 Bremen, Germany
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Received: 13 March 2019 / Revised: 3 April 2019 / Accepted: 4 April 2019 / Published: 5 April 2019
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Abstract

We report here that a straightforward change of the standard derivatization procedure for GC–MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
Keywords: metabolomics; sample preparation; gas chromatography; derivatization; silylation metabolomics; sample preparation; gas chromatography; derivatization; silylation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liebeke, M.; Puskás, E. Drying Enhances Signal Intensities for Global GC–MS Metabolomics. Metabolites 2019, 9, 68.

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