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Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
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Metabolites 2019, 9(12), 296; https://doi.org/10.3390/metabo9120296
Received: 8 October 2019 / Revised: 26 November 2019 / Accepted: 2 December 2019 / Published: 5 December 2019
Apocynum plants, especially A. venetum and A. hendersonii, are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb (A. venetum) and 233.74 Mb (A. hendersonii) and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in A. venetum were found to be much higher than in A. hendersonii, and such was thought to be the reason for the morphological difference in color of A. venetum and A. hendersonii. This study provides valuable genomic and metabolome information for understanding of A. venetum and A. hendersonii, and lays a foundation for elucidating Apocynum genus plant flavonoid biosynthesis. View Full-Text
Keywords: Apocynum venetum; Apocynum hendersonii; genomic survey; flavonoids; metabolites profiles; gene expression Apocynum venetum; Apocynum hendersonii; genomic survey; flavonoids; metabolites profiles; gene expression
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Gao, G.; Chen, P.; Chen, J.; Chen, K.; Wang, X.; Abubakar, A.S.; Liu, N.; Yu, C.; Zhu, A. Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways. Metabolites 2019, 9, 296.

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