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Lomatogonium Rotatum for Treatment of Acute Liver Injury in Mice: A Metabolomics Study

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National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330002, China
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State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China
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NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 119077, Singapore
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Saw Swee Hock School of Public Health, National University of Singapore, Singapore 117549, Singapore
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NUS Environmental Research Institute, National University of Singapore, Singapore 117411, Singapore
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Authors to whom correspondence should be addressed.
Metabolites 2019, 9(10), 227; https://doi.org/10.3390/metabo9100227
Received: 10 September 2019 / Revised: 29 September 2019 / Accepted: 12 October 2019 / Published: 14 October 2019
Lomatogonium rotatum (L.) Fries ex Nym (LR) is used as a traditional Mongolian medicine to treat liver and bile diseases. This study aimed to investigate the hepatoprotective effect of LR on mice with CCl4-induced acute liver injury through conventional assays and metabolomics analysis. This study consisted of male mice (n = 23) in four groups (i.e., control, model, positive control, and LR). The extract of whole plant of LR was used to treat mice in the LR group. Biochemical and histological assays (i.e., serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), and histological changes of liver tissue) were used to evaluate LR efficacy, and metabolomics analysis based on GC-MS and LC-MS was conducted to reveal metabolic changes. The conventional analysis and metabolomic profiles both suggested that LR treatment could protect mice against CCl4-induced acute liver injury. The affected metabolic pathways included linoleic acid metabolism, α-linolenic acid metabolism, arachidonic acid metabolism, CoA biosynthesis, glycerophospholipid metabolism, the TCA cycle, and purine metabolism. This study identified eight metabolites, including phosphopantothenic acid, succinic acid, AMP, choline, glycerol 3-phosphate, linoleic acid, arachidonic acid, and DHA, as potential biomarkers for evaluating hepatoprotective effect of LR. This metabolomics study may shed light on possible mechanisms of hepatoprotective effect of LR. View Full-Text
Keywords: Lomatogonium rotatum; metabolomics; acute liver injury; LC-MS; GC-MS Lomatogonium rotatum; metabolomics; acute liver injury; LC-MS; GC-MS
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Chen, R.; Wang, Q.; Zhao, L.; Yang, S.; Li, Z.; Feng, Y.; Chen, J.; Ong, C.N.; Zhang, H. Lomatogonium Rotatum for Treatment of Acute Liver Injury in Mice: A Metabolomics Study. Metabolites 2019, 9, 227.

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