Dynamics of Monoterpene Formation in Spike Lavender Plants
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Departamento de Biología Vegetal, ISIC/ERI de Biotecnología y Biomedicina BIOTECMED, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain
2
Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Germany
*
Author to whom correspondence should be addressed.
Metabolites 2017, 7(4), 65; https://doi.org/10.3390/metabo7040065
Received: 24 October 2017
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Revised: 15 December 2017
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Accepted: 16 December 2017
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Published: 19 December 2017
(This article belongs to the Special Issue Isotope Guided Metabolomics and Flux Analysis)
The metabolic cross-talk between the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways was analyzed in spike lavender (Lavandula latifolia Med) on the basis of 13CO2-labelling experiments using wildtype and transgenic plants overexpressing the 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), the first and key enzyme of the MVA pathway. The plants were labelled in the presence of 13CO2 in a gas chamber for controlled pulse and chase periods of time. GC/MS and NMR analysis of 1,8-cineole and camphor, the major monoterpenes present in their essential oil, indicated that the C5-precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of both monoterpenes are predominantly biosynthesized via the MEP pathway. Surprisingly, overexpression of HMGR did not have significant impact upon the crosstalk between the MVA and MEP pathways indicating that the MEP route is the preferred pathway for the synthesis of C5 monoterpene precursors in spike lavender.
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Keywords:
essential oils; isotopologue profiling; lamiaceae; Lavandula latifolia; spike lavender; terpenoid biosynthesis; mevalonate; CO2
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MDPI and ACS Style
Mendoza-Poudereux, I.; Kutzner, E.; Huber, C.; Segura, J.; Arrillaga, I.; Eisenreich, W. Dynamics of Monoterpene Formation in Spike Lavender Plants. Metabolites 2017, 7, 65. https://doi.org/10.3390/metabo7040065
AMA Style
Mendoza-Poudereux I, Kutzner E, Huber C, Segura J, Arrillaga I, Eisenreich W. Dynamics of Monoterpene Formation in Spike Lavender Plants. Metabolites. 2017; 7(4):65. https://doi.org/10.3390/metabo7040065
Chicago/Turabian StyleMendoza-Poudereux, Isabel, Erika Kutzner, Claudia Huber, Juan Segura, Isabel Arrillaga, and Wolfgang Eisenreich. 2017. "Dynamics of Monoterpene Formation in Spike Lavender Plants" Metabolites 7, no. 4: 65. https://doi.org/10.3390/metabo7040065
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