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Monoacylglycerol Analysis Using MS/MSALL Quadruple Time of Flight Mass Spectrometry

BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Author to whom correspondence should be addressed.
Academic Editors: Vladimir Tolstikov and Peter Meikle
Metabolites 2016, 6(3), 25;
Received: 16 June 2016 / Revised: 9 August 2016 / Accepted: 12 August 2016 / Published: 17 August 2016
PDF [2960 KB, uploaded 17 August 2016]


Monoacylglycerols (MAGs) are structural and bioactive metabolites critical for biological function. Development of facile tools for measuring MAG are essential to understand its role in different diseases and various pathways. A data-independent acquisition method, MS/MSALL, using electrospray ionization (ESI) coupled quadrupole time of flight mass spectrometry (MS), was utilized for the structural identification and quantitative analysis of individual MAG molecular species. Compared with other acylglycerols, diacylglycerols (DAG) and triacylglycerols (TAG), MAG characteristically presented as a dominant protonated ion, [M + H]+, and under low collision energy as fatty acid-like fragments due to the neutral loss of the glycerol head group. At low concentrations (<10 pmol/µL), where lipid-lipid interactions are rare, there was a strong linear correlation between ion abundance and MAG concentration. Moreover, using the MS/MSALL method the major MAG species from human plasma and mouse brown and white adipose tissues were quantified in less than 6 min. Collectively, these results demonstrate that MS/MSALL analysis of MAG is an enabling strategy for the direct identification and quantitative analysis of low level MAG species from biological samples with high throughput and sensitivity. View Full-Text
Keywords: monoacyglycerols; MS/MSALL; mass spectrometry; QTOF; acylglycerols monoacyglycerols; MS/MSALL; mass spectrometry; QTOF; acylglycerols

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Gao, F.; McDaniel, J.; Chen, E.Y.; Rockwell, H.; Lynes, M.D.; Tseng, Y.-H.; Sarangarajan, R.; Narain, N.R.; Kiebish, M.A. Monoacylglycerol Analysis Using MS/MSALL Quadruple Time of Flight Mass Spectrometry. Metabolites 2016, 6, 25.

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