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Communication

Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis

1
Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan
2
Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Peter Meikle
Metabolites 2016, 6(1), 3; https://doi.org/10.3390/metabo6010003
Received: 2 December 2015 / Revised: 19 December 2015 / Accepted: 4 January 2016 / Published: 12 January 2016
Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration. View Full-Text
Keywords: Plasma-free amino acid; pre-column labeling; liquid chromatography measurements; canine plasma amino acid levels; pre- and post-food intake Plasma-free amino acid; pre-column labeling; liquid chromatography measurements; canine plasma amino acid levels; pre- and post-food intake
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MDPI and ACS Style

Azuma, K.; Hirao, Y.; Hayakawa, Y.; Murahata, Y.; Osaki, T.; Tsuka, T.; Imagawa, T.; Okamoto, Y.; Ito, N. Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites 2016, 6, 3. https://doi.org/10.3390/metabo6010003

AMA Style

Azuma K, Hirao Y, Hayakawa Y, Murahata Y, Osaki T, Tsuka T, Imagawa T, Okamoto Y, Ito N. Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites. 2016; 6(1):3. https://doi.org/10.3390/metabo6010003

Chicago/Turabian Style

Azuma, Kazuo, Yoshiko Hirao, Yoshihiro Hayakawa, Yusuke Murahata, Tomohiro Osaki, Takeshi Tsuka, Tomohiro Imagawa, Yoshiharu Okamoto, and Norihiko Ito. 2016. "Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis" Metabolites 6, no. 1: 3. https://doi.org/10.3390/metabo6010003

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