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Keywords = canine plasma amino acid levels

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10 pages, 1472 KiB  
Communication
Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis
by Kazuo Azuma, Yoshiko Hirao, Yoshihiro Hayakawa, Yusuke Murahata, Tomohiro Osaki, Takeshi Tsuka, Tomohiro Imagawa, Yoshiharu Okamoto and Norihiko Ito
Metabolites 2016, 6(1), 3; https://doi.org/10.3390/metabo6010003 - 12 Jan 2016
Cited by 15 | Viewed by 6110
Abstract
Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column [...] Read more.
Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration. Full article
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