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Article

Multi-Spectroscopic Analysis of Seed Quality and 13C-Stable-Iotopologue Monitoring in Initial Growth Metabolism of Jatropha curcas L.

1
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
2
Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
3
Faculty of Agriculture, Tottori University, 4-101 Koyama-cho, Tottori 680-8553, Japan
4
RIKEN Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
5
Graduate School of Bioagricultural Sciences, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya, Aichi 464-0810, Japan
*
Author to whom correspondence should be addressed.
Metabolites 2014, 4(4), 1018-1033; https://doi.org/10.3390/metabo4041018
Received: 28 April 2014 / Revised: 10 September 2014 / Accepted: 5 November 2014 / Published: 13 November 2014
(This article belongs to the Special Issue Metabolomics and Biotechnology)
In the present study, we applied nuclear magnetic resonance (NMR), as well as near-infrared (NIR) spectroscopy, to Jatropha curcas to fulfill two objectives: (1) to qualitatively examine the seeds stored at different conditions, and (2) to monitor the metabolism of J. curcas during its initial growth stage under stable-isotope-labeling condition (until 15 days after seeding). NIR spectra could non-invasively distinguish differences in storage conditions. NMR metabolic analysis of water-soluble metabolites identified sucrose and raffinose family oligosaccharides as positive markers and gluconic acid as a negative marker of seed germination. Isotopic labeling patteren of metabolites in germinated seedlings cultured in agar-plate containg 13C-glucose and 15N-nitrate was analyzed by zero-quantum-filtered-total correlation spectroscopy (ZQF-TOCSY) and 13C-detected 1H-13C heteronuclear correlation spectroscopy (HETCOR). 13C-detected HETOCR with 13C-optimized cryogenic probe provided high-resolution 13C-NMR spectra of each metabolite in molecular crowd. The 13C-13C/12C bondmer estimated from 1H-13C HETCOR spectra indicated that glutamine and arginine were the major organic compounds for nitrogen and carbon transfer from roots to leaves. View Full-Text
Keywords: NMR; stable-isotope labeling; quality examination; isotopic analysis NMR; stable-isotope labeling; quality examination; isotopic analysis
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MDPI and ACS Style

Komatsu, T.; Ohishi, R.; Shino, A.; Akashi, K.; Kikuchi, J. Multi-Spectroscopic Analysis of Seed Quality and 13C-Stable-Iotopologue Monitoring in Initial Growth Metabolism of Jatropha curcas L. Metabolites 2014, 4, 1018-1033. https://doi.org/10.3390/metabo4041018

AMA Style

Komatsu T, Ohishi R, Shino A, Akashi K, Kikuchi J. Multi-Spectroscopic Analysis of Seed Quality and 13C-Stable-Iotopologue Monitoring in Initial Growth Metabolism of Jatropha curcas L. Metabolites. 2014; 4(4):1018-1033. https://doi.org/10.3390/metabo4041018

Chicago/Turabian Style

Komatsu, Takanori, Risa Ohishi, Amiu Shino, Kinya Akashi, and Jun Kikuchi. 2014. "Multi-Spectroscopic Analysis of Seed Quality and 13C-Stable-Iotopologue Monitoring in Initial Growth Metabolism of Jatropha curcas L." Metabolites 4, no. 4: 1018-1033. https://doi.org/10.3390/metabo4041018

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