Next Article in Journal
NblA1/A2-Dependent Homeostasis of Amino Acid Pools during Nitrogen Starvation in Synechocystis sp. PCC 6803
Next Article in Special Issue
Insect-Induced Daidzein, Formononetin and Their Conjugates in Soybean Leaves
Previous Article in Journal
Breath Analysis in Disease Diagnosis: Methodological Considerations and Applications
Previous Article in Special Issue
Reliable Metabolic Flux Estimation in Escherichia coli Central Carbon Metabolism Using Intracellular Free Amino Acids
Open AccessArticle

Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
*
Author to whom correspondence should be addressed.
Metabolites 2014, 4(2), 499-516; https://doi.org/10.3390/metabo4020499
Received: 17 April 2014 / Revised: 5 June 2014 / Accepted: 12 June 2014 / Published: 20 June 2014
(This article belongs to the Special Issue Metabolomics and Biotechnology)
Recently, cyanobacteria have become one of the most attractive hosts for biochemical production due to its high proliferative ability and ease of genetic manipulation. Several researches aimed at biological production using modified cyanobacteria have been reported previously. However, to improve the yield of bioproducts, a thorough understanding of the intercellular metabolism of cyanobacteria is necessary. Metabolic profiling techniques have proven to be powerful tools for monitoring cellular metabolism of various organisms and can be applied to elucidate the details of cyanobacterial metabolism. In this study, we constructed a metabolic profiling method for cyanobacteria using 13C-labeled cell extracts as internal standards. Using this method, absolute concentrations of 84 metabolites were successfully determined in three cyanobacterial strains which are commonly used as background strains for metabolic engineering. By comparing the differences in basic metabolic potentials of the three cyanobacterial strains, we found a well-correlated relationship between intracellular energy state and growth in cyanobacteria. By integrating our results with the previously reported biological production pathways in cyanobacteria, we found putative limiting step of carbon flux. The information obtained from this study will not only help gain insights in cyanobacterial physiology but also serve as a foundation for future metabolic engineering studies using cyanobacteria. View Full-Text
Keywords: metabolomics; cyanobacteria; biological production; absolute quantitation; triple quadrupole mass spectrometry metabolomics; cyanobacteria; biological production; absolute quantitation; triple quadrupole mass spectrometry
Show Figures

Figure 1

MDPI and ACS Style

Dempo, Y.; Ohta, E.; Nakayama, Y.; Bamba, T.; Fukusaki, E. Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production. Metabolites 2014, 4, 499-516.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop