Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Sampling
2.2. Sample Preparation and Extraction
2.3. Quadrupole-Orbitrap UHPLC–MS/MS Analysis
2.4. Comprehensive Metabolomic Analysis of Coconut Fruit
2.5. Metabolome Data Analysis
2.6. RNA Sequencing and Data Analysis
2.7. Weighted Correlation Network Analysis and Gene Network Visualization
3. Results
3.1. Metabolome Profiling of Different Coconut Cultivars
3.2. Transcriptome Analysis of Different Coconut Cultivars
3.3. Analysis of Glutathione Metabolic Pathways in Coconuts
3.4. Regulatory Mechanisms of Differential Polyamine Metabolism
3.5. WGCNA Reveals Regulatory Networks Related to α-Linolenate
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guo, H.; Li, C.; Lai, J.; Tong, H.; Cao, Z.; Wang, C.; Zhao, W.; He, L.; Wang, S.; Yang, J.; et al. Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients. Metabolites 2023, 13, 683. https://doi.org/10.3390/metabo13060683
Guo H, Li C, Lai J, Tong H, Cao Z, Wang C, Zhao W, He L, Wang S, Yang J, et al. Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients. Metabolites. 2023; 13(6):683. https://doi.org/10.3390/metabo13060683
Chicago/Turabian StyleGuo, Hao, Chun Li, Jun Lai, Haiyang Tong, Zhenfeng Cao, Chao Wang, Wenyu Zhao, Liqiang He, Shouchuang Wang, Jun Yang, and et al. 2023. "Comprehensive Analysis of Metabolome and Transcriptome Reveals the Regulatory Network of Coconut Nutrients" Metabolites 13, no. 6: 683. https://doi.org/10.3390/metabo13060683