Anti-Inflammatory Activity of Compounds Derived from Vitex rotundifolia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Extraction and Separation
2.3. Modified Mosher’s Method
Spectroscopic Data of MTPA Diester Derivatives
2.4. Antioxidant Assay
2.5. NO Assay
2.5.1. Cell Culture and Viability
2.5.2. Measurement of NO Production
2.6. IL-8 Assay
2.6.1. Cell Culture and Viability
2.6.2. Measurement of IL-8 Production
2.7. In silico Assay
2.8. Statistical Analysis
3. Results
3.1. Separation and Identification of Chemical Constituents
3.2. Structural Determination of Compounds (1−20)
3.3. Biological Activities of Isolated Compounds
3.4. In silico Approach Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | 1 | |
---|---|---|
δC | δH (mult., J = Hz) | |
1 | 128.2 | - |
2 | 157.1 | - |
3 | 43.4 | 2.42 (1H, d, 19.0) 2.84 (1H, dd, 5.5, 19.0) |
4 | 72.4 | 4.01 (1H, d, 6.0) |
5 | 53.1 | 2.82 (1H, d, 5.5) |
6 | 166.6 | - |
7 | 58.9 | 4.40 (2H, brs) |
8 | 30.2 | 1.62 (1H, ddd, 4.8, 9.4, 14.2) 1.94 (1H, td, 3.7, 7.1, 14.2) |
9 | 62.8 | 4.18 (1H, m) 4.25 (1H, m) |
1’ | 120.5 | - |
2’/6’ | 131.4 | 7.81 (2H, d, 8.7) |
3’/5’ | 115.3 | 6.84 (2H, d, 8.7) |
4’ | 161.9 | - |
7’ | 165.6 | - |
Compounds | Docking Score (kcal/mol) | Conventional Hydrogen Bond | Other Interactions |
---|---|---|---|
8 | −7.5 | Gly44, Glu46 | Gln6, Lys9, Thr10, Tyr11, Arg45, Leu47 |
9 | −6.6 | Gln6, Lys9, Arg24 | Thr10, Tyr11, Ile38, Glu46, Leu47, Cys48 |
13 | −8.3 | Gln6, Ile8, Ser12, Lys13, Cys48 | Cys7, Lys9, Thr10, Tyr11, Ile38, Glu46Leu47 |
14 | −8.4 | Lys9, Cys48 | Arg4, Cys5, Gln6, Ile8, Thr10, Tyr11, Ile38, Glu46, Leu47 |
19 | −7.0 | Gln57 | Val25, Glu27, Ser28, Thr35, Ile37, Pro51, Lys52, Val60, Glu61 |
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Le, D.; Han, S.; Min, K.H.; Lee, M. Anti-Inflammatory Activity of Compounds Derived from Vitex rotundifolia. Metabolites 2023, 13, 249. https://doi.org/10.3390/metabo13020249
Le D, Han S, Min KH, Lee M. Anti-Inflammatory Activity of Compounds Derived from Vitex rotundifolia. Metabolites. 2023; 13(2):249. https://doi.org/10.3390/metabo13020249
Chicago/Turabian StyleLe, DucDat, Sanghee Han, Kyung Hyun Min, and Mina Lee. 2023. "Anti-Inflammatory Activity of Compounds Derived from Vitex rotundifolia" Metabolites 13, no. 2: 249. https://doi.org/10.3390/metabo13020249