Guanosine Prevents Spatial Memory Impairment and Hippocampal Damage Following Amyloid-β1–42 Administration in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Drugs
2.3. Amyloid-Beta Infusion
2.4. Experimental Design
2.5. Behavioral Analysis
2.6. Tail Suspension Test (TST)
2.7. Open-Field Test (OFT)
2.8. Sucrose Splash Test (SST)
2.9. Object Location Task (OLT)
2.10. Biochemistry Analysis
2.10.1. Preparation of Brain Slices
2.10.2. Cellular Viability Evaluation
2.10.3. Propidium Iodide Incorporation
2.10.4. Reactive Oxygen Species (ROS) Generation
2.10.5. Mitochondrial Membrane Potential (ΔΨm) Measurement
2.10.6. L-[3H]Glutamate Release
2.10.7. Protein Measurement
2.11. Statistical Analysis
3. Results
3.1. Guanosine Prevents Aβ1–42-Induced ROS Production
3.2. Guanosine Prevents Aβ1–42-Induced Short-Term Spatial Memory Impairment
3.3. Guanosine Prevents Aβ1–42-Induced Hippocampal Slice Damage
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Coelho, V.; Binder, L.B.; Marques, N.F.; Constantino, L.C.; Mancini, G.; Tasca, C.I. Guanosine Prevents Spatial Memory Impairment and Hippocampal Damage Following Amyloid-β1–42 Administration in Mice. Metabolites 2022, 12, 1207. https://doi.org/10.3390/metabo12121207
Coelho V, Binder LB, Marques NF, Constantino LC, Mancini G, Tasca CI. Guanosine Prevents Spatial Memory Impairment and Hippocampal Damage Following Amyloid-β1–42 Administration in Mice. Metabolites. 2022; 12(12):1207. https://doi.org/10.3390/metabo12121207
Chicago/Turabian StyleCoelho, Victor, Luisa Bandeira Binder, Naiani Ferreira Marques, Leandra Celso Constantino, Gianni Mancini, and Carla Inês Tasca. 2022. "Guanosine Prevents Spatial Memory Impairment and Hippocampal Damage Following Amyloid-β1–42 Administration in Mice" Metabolites 12, no. 12: 1207. https://doi.org/10.3390/metabo12121207