Next Article in Journal
Multi-Omics-Based Discovery of Plant Signaling Molecules
Next Article in Special Issue
Effects of Light and Temperature on the Metabolic Profiling of Two Habitat-Dependent Bloom-Forming Cyanobacteria
Previous Article in Journal
Relationship of Inflammatory Markers and Metabolic Syndrome in Postmenopausal Women
 
 
Review

Kinetic Modeling of Saccharomyces cerevisiae Central Carbon Metabolism: Achievements, Limitations, and Opportunities

1
Department of Biomedical Engineering, Eindhoven University of Technology, Groene Loper 5, 5612 AE Eindhoven, The Netherlands
2
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands
3
DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands
4
Systems Biology Lab, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, 1081 HZ Amsterdam, The Netherlands
5
Lehrstuhl für Bioverfahrenstechnik, FAU Erlangen-Nürnberg, 91052 Erlangen, Germany
6
Amsterdam University Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: David J. Beale
Metabolites 2022, 12(1), 74; https://doi.org/10.3390/metabo12010074
Received: 8 December 2021 / Revised: 11 January 2022 / Accepted: 12 January 2022 / Published: 13 January 2022
(This article belongs to the Special Issue Metabolic Functionality of Microorganisms under Dynamic Environments)
Central carbon metabolism comprises the metabolic pathways in the cell that process nutrients into energy, building blocks and byproducts. To unravel the regulation of this network upon glucose perturbation, several metabolic models have been developed for the microorganism Saccharomyces cerevisiae. These dynamic representations have focused on glycolysis and answered multiple research questions, but no commonly applicable model has been presented. This review systematically evaluates the literature to describe the current advances, limitations, and opportunities. Different kinetic models have unraveled key kinetic glycolytic mechanisms. Nevertheless, some uncertainties regarding model topology and parameter values still limit the application to specific cases. Progressive improvements in experimental measurement technologies as well as advances in computational tools create new opportunities to further extend the model scale. Notably, models need to be made more complex to consider the multiple layers of glycolytic regulation and external physiological variables regulating the bioprocess, opening new possibilities for extrapolation and validation. Finally, the onset of new data representative of individual cells will cause these models to evolve from depicting an average cell in an industrial fermenter, to characterizing the heterogeneity of the population, opening new and unseen possibilities for industrial fermentation improvement. View Full-Text
Keywords: yeast; central metabolism; stress response; metabolic regulation; kinetic model; in vivo kinetics; parameter estimation; complexity; uncertainty; population heterogeneity yeast; central metabolism; stress response; metabolic regulation; kinetic model; in vivo kinetics; parameter estimation; complexity; uncertainty; population heterogeneity
Show Figures

Figure 1

MDPI and ACS Style

Lao-Martil, D.; Verhagen, K.J.A.; Schmitz, J.P.J.; Teusink, B.; Wahl, S.A.; van Riel, N.A.W. Kinetic Modeling of Saccharomyces cerevisiae Central Carbon Metabolism: Achievements, Limitations, and Opportunities. Metabolites 2022, 12, 74. https://doi.org/10.3390/metabo12010074

AMA Style

Lao-Martil D, Verhagen KJA, Schmitz JPJ, Teusink B, Wahl SA, van Riel NAW. Kinetic Modeling of Saccharomyces cerevisiae Central Carbon Metabolism: Achievements, Limitations, and Opportunities. Metabolites. 2022; 12(1):74. https://doi.org/10.3390/metabo12010074

Chicago/Turabian Style

Lao-Martil, David, Koen J. A. Verhagen, Joep P. J. Schmitz, Bas Teusink, S. Aljoscha Wahl, and Natal A. W. van Riel. 2022. "Kinetic Modeling of Saccharomyces cerevisiae Central Carbon Metabolism: Achievements, Limitations, and Opportunities" Metabolites 12, no. 1: 74. https://doi.org/10.3390/metabo12010074

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop