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Article

Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry

1
Department of Gastrointestinal and Pediatric Surgery, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Shinjukuku, Tokyo 160-0023, Japan
2
Department of Surgery, Kohsei Chuo General Hospital, 1-11-27 Mita, Meguroku, Tokyo 153-0062, Japan
3
Department of Gastroenterology, Kohsei Chuo General Hospital, 1-11-27 Mita, Meguroku, Tokyo 153-0062, Japan
4
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0811, Japan
5
Research and Development Center for Minimally Invasive Therapies, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjukuku, Tokyo 160-0022, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Helen G. Gika and Thusitha W. Rupasinghe
Metabolites 2022, 12(1), 59; https://doi.org/10.3390/metabo12010059
Received: 15 November 2021 / Revised: 25 December 2021 / Accepted: 6 January 2022 / Published: 10 January 2022
(This article belongs to the Section Metabolomic Profiling Technology)
This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between patients with CRC and healthy controls (HC). We analyzed additional samples and confirmed consistency between the newly and previously analyzed data. In total, we included 36 HC, 34 adenoma (AD), and 214 CRC samples, which were used for subsequent analyses. Among the 132 quantified metabolites, 16 exhibited consistent differences in both datasets, which included polyamines, etc. Pathway analyses of the integrated data revealed significant differences in many metabolites, such as glutamine, and metabolites of the TCA (tricarboxylic acid cycle) and urea cycles. The discrimination ability of the combination of multiple metabolites among the three groups was evaluated, which yielded higher sensitivity than tumor markers. The Mann–Whitney test was employed to evaluate the prognosis predictivity of the assessed metabolites and the difference between the patients with or without recurrence, which yielded 16 significantly different metabolites. Among these 16 metabolites, 11 presented significant prognosis predictivity. These data indicated the potential of metabolite-based discrimination of patients with CRC and AD from HC and prognosis predictivity of the monitored metabolites. View Full-Text
Keywords: colorectal cancer; adenoma; capillary electrophoresis-mass spectrometry; metabolome colorectal cancer; adenoma; capillary electrophoresis-mass spectrometry; metabolome
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MDPI and ACS Style

Sakurai, T.; Katsumata, K.; Udo, R.; Tago, T.; Kasahara, K.; Mazaki, J.; Kuwabara, H.; Kawakita, H.; Enomoto, M.; Ishizaki, T.; Nemoto, Y.; Osaka, Y.; Nagakawa, Y.; Sugimoto, M.; Tsuchida, A. Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry. Metabolites 2022, 12, 59. https://doi.org/10.3390/metabo12010059

AMA Style

Sakurai T, Katsumata K, Udo R, Tago T, Kasahara K, Mazaki J, Kuwabara H, Kawakita H, Enomoto M, Ishizaki T, Nemoto Y, Osaka Y, Nagakawa Y, Sugimoto M, Tsuchida A. Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry. Metabolites. 2022; 12(1):59. https://doi.org/10.3390/metabo12010059

Chicago/Turabian Style

Sakurai, Toru, Kenji Katsumata, Ryutaro Udo, Tomoya Tago, Kenta Kasahara, Junichi Mazaki, Hiroshi Kuwabara, Hideaki Kawakita, Masanobu Enomoto, Tetsuo Ishizaki, Yukako Nemoto, Yoshiaki Osaka, Yuichi Nagakawa, Masahiro Sugimoto, and Akihiko Tsuchida. 2022. "Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry" Metabolites 12, no. 1: 59. https://doi.org/10.3390/metabo12010059

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