Lentinan Protects against Nonalcoholic Fatty Liver Disease by Reducing Oxidative Stress and Apoptosis via the PPARα Pathway
Abstract
:1. Introduction
2. Results
2.1. LNT Prevented HFD-Induced Steatosis in Mice
2.2. LNT Protected against HFD-Induced Hepatic Oxidative Stress and Apoptosis in Mice
2.3. LNT Prevented Lipid Accumulation in PA-Induced AML12 Cells
2.4. LNT Improved Oxidative Stress and Apoptosis in PA-Induced AML12 Cells
2.5. PPARα Knockdown Abolished the Protective Effects of LNT on Lipid Deposition in PA-Induced AML12 Cells
2.6. PPARα Knockdown Prevented the Effects of LNT on Oxidative Stress and Apoptosis in PA-Induced AML12 Cells
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Cell Culture and Treatments
4.3. Biochemical Parameters
4.4. Western Blot Analysis
4.5. Histological Analysis
4.6. Flow Cytometry Analysis
4.7. Quantitative RT-PCR
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Du, T.; Fang, Q.; Zhang, Z.; Zhu, C.; Xu, R.; Chen, G.; Wang, Y. Lentinan Protects against Nonalcoholic Fatty Liver Disease by Reducing Oxidative Stress and Apoptosis via the PPARα Pathway. Metabolites 2022, 12, 55. https://doi.org/10.3390/metabo12010055
Du T, Fang Q, Zhang Z, Zhu C, Xu R, Chen G, Wang Y. Lentinan Protects against Nonalcoholic Fatty Liver Disease by Reducing Oxidative Stress and Apoptosis via the PPARα Pathway. Metabolites. 2022; 12(1):55. https://doi.org/10.3390/metabo12010055
Chicago/Turabian StyleDu, Tingyi, Qin Fang, Zhihao Zhang, Chuanmeng Zhu, Renfan Xu, Guangzhi Chen, and Yan Wang. 2022. "Lentinan Protects against Nonalcoholic Fatty Liver Disease by Reducing Oxidative Stress and Apoptosis via the PPARα Pathway" Metabolites 12, no. 1: 55. https://doi.org/10.3390/metabo12010055
APA StyleDu, T., Fang, Q., Zhang, Z., Zhu, C., Xu, R., Chen, G., & Wang, Y. (2022). Lentinan Protects against Nonalcoholic Fatty Liver Disease by Reducing Oxidative Stress and Apoptosis via the PPARα Pathway. Metabolites, 12(1), 55. https://doi.org/10.3390/metabo12010055