Next Article in Journal
The Proteomic Signature of Intestinal Acute Rejection in the Mouse
Next Article in Special Issue
Mycorrhiza-Tree-Herbivore Interactions: Alterations in Poplar Metabolome and Volatilome
Previous Article in Journal
Cold Exposure during the Active Phase Affects the Short-Chain Fatty Acid Production of Mice in a Time-Specific Manner
Previous Article in Special Issue
Species- and Caste-Specific Gut Metabolomes in Fungus-Farming Termites
Article

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

1
Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
2
National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
3
Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan
4
Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
5
Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada
*
Authors to whom correspondence should be addressed.
Academic Editors: Nicole van Dam and Remington X. Poulin
Metabolites 2022, 12(1), 22; https://doi.org/10.3390/metabo12010022
Received: 11 November 2021 / Revised: 22 December 2021 / Accepted: 25 December 2021 / Published: 27 December 2021
(This article belongs to the Special Issue Metabolomics in Chemical Ecology)
Actinobacteria are a group of ecologically important bacteria capable of producing diverse bioactive compounds. However, much remains unknown about the taxonomic and metabolic diversities of actinobacteria from many geographic regions and ecological niches. In this study, we report the isolation of actinobacteria from moss and moss-associated rhizosphere soils in Thailand. Among the 89 isolates analyzed for their bioactivities, 86 strains produced indole-3-acetic acid (IAA, ranging from 0.04 to 59.12 mg/L); 42 strains produced hydroxamate type of siderophore; 35 strains produced catecholate type of siderophore; 21 strains solubilized tricalcium phosphate; and many strains exhibited antagonistic activities against one to several of the seven selected plant, animal, and human pathogens. Overall, actinobacteria from the rhizosphere soil of mosses showed greater abilities to produce IAA and siderophores and to solubilize tricalcium phosphate than those from mosses. Among these 89 isolates, 37 were analyzed for their 16S rRNA gene sequences, which revealed their diverse phylogenetic distributions among seven genera, Streptomyces, Micromonospora, Nocardia, Actinoplanes, Saccharothrix, Streptosporangium, and Cryptosporangium. Furthermore, gas chromatography-mass spectrometry analyses of ethyl acetate crude extracts of three selected isolates with inhibitory effects against a methicillin-resistant Staphylococcus aureus strain revealed diverse metabolites with known antimicrobial activities. Together, our results demonstrate that actinobacteria from mosses in Thailand are taxonomically diverse and capable of producing a range of metabolites with plant-growth-promoting and microbial pathogen-inhibiting potentials. View Full-Text
Keywords: actinobacteria; moss; selective isolation; plant growth promotion; MRSA (methicillin-resistant Staphylococcus aureus); white-nose syndrome; VOCs (volatile organic compounds); GC-MS (gas chromatography–mass spectrometry) actinobacteria; moss; selective isolation; plant growth promotion; MRSA (methicillin-resistant Staphylococcus aureus); white-nose syndrome; VOCs (volatile organic compounds); GC-MS (gas chromatography–mass spectrometry)
Show Figures

Graphical abstract

MDPI and ACS Style

Insuk, C.; Pongpamorn, P.; Forsythe, A.; Matsumoto, A.; Ōmura, S.; Pathom-aree, W.; Cheeptham, N.; Xu, J. Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand. Metabolites 2022, 12, 22. https://doi.org/10.3390/metabo12010022

AMA Style

Insuk C, Pongpamorn P, Forsythe A, Matsumoto A, Ōmura S, Pathom-aree W, Cheeptham N, Xu J. Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand. Metabolites. 2022; 12(1):22. https://doi.org/10.3390/metabo12010022

Chicago/Turabian Style

Insuk, Chadabhorn, Pornkanok Pongpamorn, Adrian Forsythe, Atsuko Matsumoto, Satoshi Ōmura, Wasu Pathom-aree, Naowarat Cheeptham, and Jianping Xu. 2022. "Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand" Metabolites 12, no. 1: 22. https://doi.org/10.3390/metabo12010022

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop