Next Article in Journal
Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus
Next Article in Special Issue
Metabolite Profiling Reveals Distinct Modulation of Complex Metabolic Networks in Non-Pigmented, Black, and Red Rice (Oryza sativa L.) Cultivars
Previous Article in Journal
Improved One-Class Modeling of High-Dimensional Metabolomics Data via Eigenvalue-Shrinkage
Previous Article in Special Issue
Effects of Long-Term Nitrogen Fertilization on the Formation of Metabolites Related to Tea Quality in Subtropical China
 
 
Article

A Medicago truncatula Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues

by 1,2,3, 3,4, 3,4,5,6 and 1,2,3,*
1
Department of Biochemistry, University of Missouri-Columbia, Columbia, MO 65211, USA
2
Metabolomics Center, University of Missouri-Columbia, Columbia, MO 65211, USA
3
Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
4
Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO 65211, USA
5
MU Data Science and Informatics Institute, University of Missouri, Columbia, MO 65211, USA
6
Department of Health Management and Informatics, School of Medicine, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Sandor Gonda
Metabolites 2021, 11(4), 238; https://doi.org/10.3390/metabo11040238
Received: 31 December 2020 / Revised: 29 March 2021 / Accepted: 6 April 2021 / Published: 13 April 2021
(This article belongs to the Special Issue Plant Metabolomics II)
Plant roots are composed of many differentiated tissue types, with each tissue exhibiting differential quantitative and qualitative accumulation of metabolites. The large-scale nontargeted metabolite profiles of these differentiated tissues are complex, which complicates the interpretation and development of hypotheses relative to the biological roles of differentially localized metabolites. Thus, we created a data visualization tool to aid in the visualization and understanding of differential metabolite accumulations in Medicago truncatula roots. This was achieved through the development of the Medicago truncatula Metabolite Atlas based upon an adaptation of the Arabidopsis Electronic Fluorescent Pictograph (eFP) Browser. Medicago truncatula roots were dissected into border cells, root cap, elongation zone, mature root, and root secretions. Each tissue was then analyzed by UHPLC-QTOF-MS and GC-Q-MS. Data were uploaded into a MySQL database and displayed in the Medicago truncatula Metabolite Atlas. The data revealed unique differential spatial localization of many metabolites, some of which are discussed here. Ultimately, the Medicago truncatula Metabolite Atlas compiles metabolite data into a singular, useful, and publicly available web-based tool that enables the visualization and understanding of differential metabolite accumulation and spatial localization. View Full-Text
Keywords: metabolomics; roots; rhizosphere; Medicago truncatula; border cells; Metabolite Atlas metabolomics; roots; rhizosphere; Medicago truncatula; border cells; Metabolite Atlas
Show Figures

Graphical abstract

MDPI and ACS Style

Kranawetter, C.; Zeng, S.; Joshi, T.; Sumner, L.W. A Medicago truncatula Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues. Metabolites 2021, 11, 238. https://doi.org/10.3390/metabo11040238

AMA Style

Kranawetter C, Zeng S, Joshi T, Sumner LW. A Medicago truncatula Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues. Metabolites. 2021; 11(4):238. https://doi.org/10.3390/metabo11040238

Chicago/Turabian Style

Kranawetter, Clayton, Shuai Zeng, Trupti Joshi, and Lloyd W. Sumner. 2021. "A Medicago truncatula Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues" Metabolites 11, no. 4: 238. https://doi.org/10.3390/metabo11040238

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop