Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes

Filiou, Michaela D.; Nussbaumer, Markus; Teplytska, Larysa; Turck, Christoph W.

doi:10.3390/metabo11020128

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Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes

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Proteomics and Biomarkers, Max Planck Institute of Psychiatry, 2-10, 80804 Munich, Germany

Department of Biological Applications and Technology, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece

Biomedical Research Division, Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas (FORTH), 45110 Ioannina, Greece

Authors to whom correspondence should be addressed.

Academic Editor: Shozo Tomonaga

Metabolites 2021, 11(2), 128; https://doi.org/10.3390/metabo11020128

Received: 12 December 2020 / Revised: 22 January 2021 / Accepted: 4 February 2021 / Published: 23 February 2021

(This article belongs to the Special Issue Metabolomics and Its Application in Human Diseases Volume 2)

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Abstract

Mouse models are widely used to study behavioral phenotypes related to neuropsychiatric disorders. However, different mouse strains vary in their inherent behavioral and molecular characteristics, which needs to be taken into account depending on the nature of the study. Here, we performed a detailed behavioral and molecular comparison of C57BL/6 (B6) and DBA/2 (DBA) mice, two inbred strains commonly used in neuropsychiatric research. We analyzed anxiety-related and depression-like traits, quantified hippocampal and plasma metabolite profiles, and assessed total antioxidant capacity (ΤAC). B6 mice exhibit increased depression-like and decreased anxiety-related behavior compared to DBA mice. Metabolite level differences indicate alterations in amino acid, nucleotide and mitochondrial metabolism that are accompanied by a decreased TAC in B6 compared to DBA mice. Our data reveal multiple behavioral and molecular differences between B6 and DBA mouse strains, which should be considered in the experimental design for phenotype, pharmacological and mechanistic studies relevant for neuropsychiatric disorders. View Full-Text

Keywords: anxiety; metabolomics; depression; psychiatry; C57BL/6; DBA/2

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Filiou, M.D.; Nussbaumer, M.; Teplytska, L.; Turck, C.W. Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes. Metabolites 2021, 11, 128. https://doi.org/10.3390/metabo11020128

AMA Style

Filiou MD, Nussbaumer M, Teplytska L, Turck CW. Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes. Metabolites. 2021; 11(2):128. https://doi.org/10.3390/metabo11020128

Chicago/Turabian Style

Filiou, Michaela D., Markus Nussbaumer, Larysa Teplytska, and Christoph W. Turck. 2021. "Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes" Metabolites 11, no. 2: 128. https://doi.org/10.3390/metabo11020128

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Behavioral and Metabolome Differences between C57BL/6 and DBA/2 Mouse Strains: Implications for Their Use as Models for Depression- and Anxiety-Like Phenotypes

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