Urinary Metabolomic Profile of Neonates Born to Women with Gestational Diabetes Mellitus
Abstract
:1. Introduction
2. Results
2.1. Clinical Characteristics of Newborns and Mothers
2.2. Urinary Concentration Values of 101 Metabolites of Newborns to Diabetic and Healthy Mothers
2.3. Multivariate Analysis
2.4. Analysis of the Maternal Urinary Metabolome during the Second and Third Trimesters of Pregnancy
3. Discussion
4. Materials and Methods
4.1. Study Design and Research Ethics Approval
4.2. Study Population
4.3. Metabolite Measurements
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | GDM | Healthy | p-Value * |
---|---|---|---|
Newborns, n (%) | 26 (54.1) | 22 (45.8) | |
Sex, n (%) b | |||
Female | 16 (61.5) | 4 (18.1) | 0.003 ** |
Male | 10 (38.4) | 18 (81.8) | |
Gestational age (weeks) a | 38.5 ± 1.3 | 38.3 ± 1.2 | 0.5 |
APGAR score, min 1 b | 8 (100.0) | 8 (100.0) | 1.00 |
APGAR score, min 5 b | 9 (100.0) | 9 (100.0) | 1.00 |
Silverman-Anderson score b | 0 (100.0) | 0 (100.0) | 1.00 |
Weight (g) a | 3026 ± 399 | 2943 ± 477 | 0.4 |
Delivery, n (%) b | |||
Vaginal | 9 (34.6) | 13 (59.1) | 0.1 |
C-section | 17 (65.4) | 9 (40.9) | |
Mothers, n (%) | 26 (54.1) | 22 (45.8) | |
Age (years) a | 28.4 ± 4.7 | 25.6 ± 2.2 | 0.05 |
Pre-BMI (Kg/m2) a | 27.87 ± 4.12 | 25.58 ± 4.22 | 0.06 |
Normal weight, n (%) b | 8 (30.8) | 10 (45.5) | 0.3 |
Overweight, n (%) b | 10 (38.5) | 9 (40.9) | 0.3 |
Obese, n (%) b | 8 (30.8) | 3 (13.6) | 0.3 |
Glucose (mg/dL) a | 86.94 ± 13.3 | 79.81 ± 8.7 | 0.03 * |
Creatinine (mg/dL) a | 0.56 ± 0.08 | 0.57 ± 0.09 | 0.6 |
Urea (mg/dL) a | 14.26 ± 4.0 | 14.19 ± 3.91 | 1.0 |
Hemoglobin (g/dL) a | 13.02 ± 1.0 | 12.81 ± 0.75 | 0.4 |
Leucocytes (×103) a | 9.01 ± 2.65 | 8.34 ± 1.70 | 0.3 |
SBP (mm Hg) a | 113.1 ± 8.7 | 108.2 ± 9.6 | 0.07 |
DBP (mm Hg) a | 74.23 ± 7.02 | 72.73 ± 7.67 | 0.5 |
Treatment, n (%) | |||
Metformin | 15 (57.7) | ||
Diet and exercise | 10 (38.5) | ||
Insulin + Metformin | 1 (3.8) |
Metabolite | Healthy Newborns | GDM Newborns | p Value |
---|---|---|---|
Median (2.5–97.5 IQR) (μM/mM Creatinine) | Median (2.5–97.5 IQR) (μM/mM Creatinine) | ||
trans-Hydroxyproline | 36.0 (8.1–96.65) | 26.4 (11.31–60.82) | 0.01 |
Glutamic acid | 7.4 (1.88–25.28) | 12.4 (3.72–36.52) | 0.01 |
DOPA | 0.06 (0.02–0.17) | 0.04 (0.01–0.08) | 0.04 |
Spermine | 0.03 (0.007–0.09) | 0.04 (0.006–0.72) | 0.003 * |
Lactic acid | 85.6 (46.95–797.5) | 112.0 (47.14–359.8) | 0.04 |
Butyric acid | 0.33 (0.12–0.9) | 0.22 (0.06–0.7) | 0.02 |
Isobutyric acid | 0.08 (0.03–1.0) | 0.05 (0.02–1.0) | 0.03 |
Glutaconylcarnitine (C5:1DC) | 0.02 (0.008–0.03) | 0.01 (0.005–0.03) | 0.009 * |
Glutarylcarnitine (C5DC) | 0.05 (0.03–0.1) | 0.04 (0.02–0.06) | 0.006 * |
C10:2 | 0.03 (0.02–0.07) | 0.03 (0.014–0.05) | 0.01 |
Hexadecenoylcarnitine (C16:1) | 0.012 (0.007–0.03) | 0.009 (0.004–0.027) | 0.01 |
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Herrera-Van Oostdam, A.S.; Salgado-Bustamante, M.; Lima-Rogel, V.; Oropeza-Valdez, J.J.; López, J.A.; Rodríguez, I.D.R.; Toro-Ortiz, J.C.; Herrera-Van Oostdam, D.A.; López-Hernández, Y.; Monárrez-Espino, J. Urinary Metabolomic Profile of Neonates Born to Women with Gestational Diabetes Mellitus. Metabolites 2021, 11, 723. https://doi.org/10.3390/metabo11110723
Herrera-Van Oostdam AS, Salgado-Bustamante M, Lima-Rogel V, Oropeza-Valdez JJ, López JA, Rodríguez IDR, Toro-Ortiz JC, Herrera-Van Oostdam DA, López-Hernández Y, Monárrez-Espino J. Urinary Metabolomic Profile of Neonates Born to Women with Gestational Diabetes Mellitus. Metabolites. 2021; 11(11):723. https://doi.org/10.3390/metabo11110723
Chicago/Turabian StyleHerrera-Van Oostdam, Ana Sofía, Mariana Salgado-Bustamante, Victoria Lima-Rogel, Juan José Oropeza-Valdez, Jesús Adrián López, Iván Daniel Román Rodríguez, Juan Carlos Toro-Ortiz, David Alejandro Herrera-Van Oostdam, Yamilé López-Hernández, and Joel Monárrez-Espino. 2021. "Urinary Metabolomic Profile of Neonates Born to Women with Gestational Diabetes Mellitus" Metabolites 11, no. 11: 723. https://doi.org/10.3390/metabo11110723