Neuronal Lipoprotein Lipase Deficiency Alters Neuronal Function and Hepatic Metabolism
Abstract
:1. Introduction
2. Results
2.1. Mice with Neuron-Specific LPL Depletion Have Improved Glucose Tolerance
2.2. LPL KD Mice Have Reduced Hepatic Glucose Production
2.3. Neuronal LPL-KD Mice Have Reduced Hepatic Lipid Accumulation
2.4. Liver-Related PVN Neurons of LPL KD Mice Have Reduced Inhibitory Synaptic Control
2.5. Loss of LPL in PVN Neurons Alters Metabolic Flux
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Glucose and Insulin Tolerance Tests
4.3. Hyperinsulinemic-Euglycemic Clamps
4.4. Analysis of Liver Lipids
4.5. Lipoprotein Profile
4.6. Quantitative Real-Time PCR
4.7. Identification of Liver-Related Neurons with PRV-152
4.8. Brain Slice Preparation
4.9. Whole-Cell Patch-Clamp Recordings
4.10. Stereotaxic AVV Injection
4.11. Fluorescence Lifetime Imaging Microscopy (FLIM)
4.12. Cell Culture Conditions and Reagents
4.13. Metabolomics
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Bruce, K.D.; Dobrinskikh, E.; Wang, H.; Rudenko, I.; Gao, H.; Libby, A.E.; Gorkhali, S.; Yu, T.; Zsombok, A.; Eckel, R.H. Neuronal Lipoprotein Lipase Deficiency Alters Neuronal Function and Hepatic Metabolism. Metabolites 2020, 10, 385. https://doi.org/10.3390/metabo10100385
Bruce KD, Dobrinskikh E, Wang H, Rudenko I, Gao H, Libby AE, Gorkhali S, Yu T, Zsombok A, Eckel RH. Neuronal Lipoprotein Lipase Deficiency Alters Neuronal Function and Hepatic Metabolism. Metabolites. 2020; 10(10):385. https://doi.org/10.3390/metabo10100385
Chicago/Turabian StyleBruce, Kimberley D., Evgenia Dobrinskikh, Hong Wang, Ivan Rudenko, Hong Gao, Andrew E. Libby, Sachi Gorkhali, Tian Yu, Andrea Zsombok, and Robert H. Eckel. 2020. "Neuronal Lipoprotein Lipase Deficiency Alters Neuronal Function and Hepatic Metabolism" Metabolites 10, no. 10: 385. https://doi.org/10.3390/metabo10100385