Chromobacterium Violaceum: A Model for Evaluating the Anti-Quorum Sensing Activities of Plant Substances
Sinosh Skariyachan
Round 1
Reviewer 1 Report
The manuscript has been evaluated and found substantial concern in the present form. The major gap in the literature and how the present manuscript mitigate the e gap and provide a new knowledge is not clear. The quorum sensing mechanism is very well studied and how the existing mechanism is different in this bacteria need to be illustrated. Here is no proper connection between the first part and the second part of the manuscript i.e, Plant Inhibitors: a new way to control bacterial communication. The link is missing. The manuscript not critically elucidated the concept mentioned in the title. There should be a greater number of figures and tables. The fig.1 is not that much impressive. Presently only one table incorporated. The balanced review require a detailed biomolecular mechanism of the pathway in which bacteria Chromobacterium violaceum that can be used as model for Evaluating Anti-Quorum Sensing Activity of Plant Substances
The manuscript need a professional proof reading with the help of an expert
Author Response
BULGARIAN ACADEMY OF SCIENCES
INSTITUTE OF MICROBIOLOGY
Acad. G. Bonchev Str., Bl. 26
1113 Sofia, Bulgaria
June 14, 2023
Dear Editor Stankovic,
Please find the re-submition of MS scipharm-2415794 entitled " Chromobacterium violaceum: Model for Evaluating Anti-Quorum Sensing Activity of Plant Substances". The MS has been corrected in accordance with the reviewers' recommendations.
We would like to thank the reviewers for their valuable comments. We include detailed answers to the reviewers' comments. The MS was subjected to major revision. All changes in the text have been labeled by “Track Changes” function. We hope the corrected version of the MS has been improved, and are ready to co-operate further.
Best regards
Corresponding author:
Tsvetelina Paunova-Krasteva, PhD
Institute of Microbiology
Bulgarian Academy of Sciences
Reply to the reviewers' comments:
REVIEWER 1
- “The quorum sensing mechanism is very well studied and how the existing mechanism is different in this bacteria need to be illustrated. Here is no proper connection between the first part and the second part of the manuscript i. e, Plant Inhibitors: a new way to control bacterial communication. The link is missing”.
Response 1:The QS mechanism of C. violaceum, CviR receptor and its function we described in part “3.1. Quorum sensing mechanisms in Chromobacterium violaceum” (p. 201-211). In the same part there is more information about QS-regulated phenotypes in C. violacein as well as the synthesis mechanism of violacein (p. 212-235). We agree with the Reviewer that the connecting information between the two parts, indeed, was limited. For this reason, we added more information in two parts 2.2. Bicomponent Quorum sensing receptors and 3.2. Pigment production, why and how C. violaceum strains are used for this type of research (p. 154-161; p. 249-253; p. 261-266).
- “There should be a greater number of figures and tables. The fig.1 is not that much impressive. Presently only one table incorporated”.
Response 2: We appreciate the suggestion of the Reviewer for including more figures and tables. In the corrected version of our MS are given detailed new information about pure plant derivatives and their mechanism on QSI activity (p. 451-481), organized in one more table (Table 2). The molecular mechanism for QS-disruption in general is mentioned in Table 1 and Table 2. Additionally, the text is supported with Figure 1.
Thank you sincerely, Reviewer 1.
Reviewer 2 Report
Dear editors
The present review demonstrated Chromobacterium violaceum as reporter organism for Evaluating Anti- Quorum Sensing Activity of Plant Substances
The MS included many QSI activities of plant extract that has been discovered using Chromobacterium violaceum
The MS still need the following points
- Focusing on some pure plant derivatives and their mechanism on QSI activity.
- The authors discussed the link between Chromobacterium violaceum and QS in Gram-negative bacteria but without supporting examples.
- How this link in applied examples utilized the Chromobacterium violaceum as reporter strain and apply the QSI of some natural products as QSI of pathogenic microbes.
- Some important references are missed.
- The effect of other AHLs on violacein pigment production needs to be discussed in details.
Dear editors
The present review demonstrated Chromobacterium violaceum as reporter organism for Evaluating Anti- Quorum Sensing Activity of Plant Substances
The MS included many QSI activities of plant extract that has been discovered using Chromobacterium violaceum
The MS still need the following points
- Focusing on some pure plant derivatives and their mechanism on QSI activity.
- The authors discussed the link between Chromobacterium violaceum and QS in Gram-negative bacteria but without supporting examples.
- How this link in applied examples utilized the Chromobacterium violaceum as reporter strain and apply the QSI of some natural products as QSI of pathogenic microbes.
- Some important references are missed.
- The effect of other AHLs on violacein pigment production needs to be discussed in details.
Author Response
REVIEWER 2
- “Focusing on some pure plant derivatives and their mechanism on QSI activity”.
Response 1: In accordance with this suggestion of the Reviewer, we are convinced that the characterization of some pure derivatives will improve the quality of manuscript. For this reason, we looked for pure plant metabolites scanned for potential QSI activity. Even though the topic is new, we manage to find some representatives. We summarized them in Table 2 and included their mechanism of action, where it was identified in p. 451-481.
- “The authors discussed the link between Chromobacterium violaceum and QS in Gram-negative bacteria but without supporting examples. How this link in applied examples utilized the Chromobacterium violaceum as reporter strain and apply the QSI of some natural products as QSI of pathogenic microbes. Some important references are missed”.
Response 2: We agree with the Reviewer that our findings should be supported by specific examples. Most of the time when a compound is tested for QSI activity, it is also tested in P. aeruginosa PAO1 and rarely, in other bacteria. We added this new information in the part “3.2 Quorum sensing inhibitory potential by plants”, plus the functionality that is influenced.
- “The effect of other AHLs on violacein pigment production needs to be discussed in details”.
Response 3: We added information about the effect of different in length AHLs over violacein synthesis, discuss in details in part 3.2. Pigment production (p. 236-241).
Thank you sincerely, Reviewer 2.
Author Response File: 
Author Response.docx
Round 2
Reviewer 1 Report
The reviewer appreciate the revision of the authors. The authors addressed most the comment. But still few more figures are required. The figures are really important to capture attention of the readers.
Need some revision
Author Response
BULGARIAN ACADEMY OF SCIENCES
INSTITUTE OF MICROBIOLOGY
Acad. G. Bonchev Str., Bl. 26
1113 Sofia, Bulgaria
June 27, 2023
Dear Editor Stankovic,
Please find the re-submition of MS scipharm-2415794 entitled "Chromobacterium violaceum: Model for Evaluating Anti-Quorum Sensing Activity of Plant Substances". The MS has been corrected in accordance with the reviewer 1 (Round 2) recommendations.
We would like to thank the reviewers for their valuable comments. The MS was subjected to major revision. In accordance with the recommendations of Reviewer 1, we included 1 new figure. All changes in the text have been labeled. The English text was edited by a professional translator, MS in English language.
We hope the corrected version of the MS has been improved, and are ready to co-operate further.
Best regards
Corresponding author:
Tsvetelina Paunova-Krasteva, PhD
Institute of Microbiology
Bulgarian Academy of Sciences
Reply to the reviewers' comments:
REVIEWER 1
The reviewer appreciate the revision of the authors. The authors addressed most the comment. But still few more figures are required. The figures are really important to capture attention of the readers.
Response: We added one more detailed figure (Figure 1) in part “3.1. Quorum sensing mechanisms in Chromobacterium violaceum”, illustrated the quorum sensing mechanism in C. violaceum (p. 219-226). Appropriate information related with QS mechanism of C. violaceum, it`s receptors and function was included in the initial version of MS in the same part (p. 198-208).
Author Response File: Author Response.docx
Round 3
Reviewer 1 Report
The authors addressed the comments'. The manuscript can be considered for publication.
Minor editing is required
