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Bioinformatics Analysis Confirms the Target Protein Underlying Mitotic Catastrophe of 4T1 Cells under Combinatorial Treatment of PGV-1 and Galangin

1
Department of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
2
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3
Macromolecular Engineering Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Murali Mohan Yallapu
Sci. Pharm. 2021, 89(3), 38; https://doi.org/10.3390/scipharm89030038
Received: 11 June 2021 / Revised: 28 July 2021 / Accepted: 29 July 2021 / Published: 10 August 2021
Pentagamavunon-1 (PGV-1), a potential chemopreventive agent with a strong cytotoxic effect, modulates prometaphase arrest. Improvement to get higher effectiveness of PGV-1 is a new challenge. A previous study reported that the natural compound, galangin, has antiproliferative activity against cancer cells with a lower cytotoxicity effect. This study aims to develop a combinatorial treatment of PGV-1 and galangin as an anticancer agent with higher effectiveness than a single agent. In this study, 4T1, a TNBC model cell, was treated with a combination of PGV-1 and galangin. As a result, PGV-1 and galangin showed a cytotoxic effect with IC50 values of 8 and 120 µM, respectively. Combining those chemicals has a synergistic impact, as shown by the combination index (CI) value of 1. Staining with the May Grunwald-Giemsa reagent indicated mitotic catastrophe evidence, characterized by micronuclear and multinucleated morphology. Moreover, the senescence percentage was higher than the single treatment. Furthermore, bioinformatics investigations showed that PGV-1 and galangin target CDK1, PLK1, and AURKB, overexpression proteins in TNBC that are essential in regulating cell cycle arrest. In conclusion, the combination of PGV-1 and galangin exhibit a synergistic effect and potential to be a chemotherapeutic drug by the mechanism of mitotic catastrophe and senescence induction. View Full-Text
Keywords: galangin; PGV-1; TNBC; mitotic catastrophe; senescence galangin; PGV-1; TNBC; mitotic catastrophe; senescence
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MDPI and ACS Style

Hasbiyani, N.A.F.; Wulandari, F.; Nugroho, E.P.; Hermawan, A.; Meiyanto, E. Bioinformatics Analysis Confirms the Target Protein Underlying Mitotic Catastrophe of 4T1 Cells under Combinatorial Treatment of PGV-1 and Galangin. Sci. Pharm. 2021, 89, 38. https://doi.org/10.3390/scipharm89030038

AMA Style

Hasbiyani NAF, Wulandari F, Nugroho EP, Hermawan A, Meiyanto E. Bioinformatics Analysis Confirms the Target Protein Underlying Mitotic Catastrophe of 4T1 Cells under Combinatorial Treatment of PGV-1 and Galangin. Scientia Pharmaceutica. 2021; 89(3):38. https://doi.org/10.3390/scipharm89030038

Chicago/Turabian Style

Hasbiyani, Nurul A.F., Febri Wulandari, Eri P. Nugroho, Adam Hermawan, and Edy Meiyanto. 2021. "Bioinformatics Analysis Confirms the Target Protein Underlying Mitotic Catastrophe of 4T1 Cells under Combinatorial Treatment of PGV-1 and Galangin" Scientia Pharmaceutica 89, no. 3: 38. https://doi.org/10.3390/scipharm89030038

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