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Open AccessArticle

Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals

Department of Pharmaceutical Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan
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Academic Editor: Chih-Chien Lin
Sci. Pharm. 2021, 89(1), 14; https://doi.org/10.3390/scipharm89010014
Received: 5 February 2021 / Revised: 25 February 2021 / Accepted: 2 March 2021 / Published: 8 March 2021
Asenapine maleate is an antipsychotic drug that is indicated in the treatment of schizophrenia and bipolar disorders. It has low aqueous solubility and high permeability (Class II drug) and undergoes an extensive first pass effect. These problems result in low oral bioavailability (<2%). To enhance its solubility/dissolution rate and hence bioavailability, co-crystals using different co-formers in different ratios were prepared and evaluated. To study the in vitro dissolution of the drug from these co-crystals into phosphate buffer (pH 6.8), an RP-HPLC method was developed and validated according to the ICH Q2R1 guidelines. The method was linear in the range 0.1–14 µg/mL (R > 0.9998) and accurate and precise. An ANOVA test indicated that calibration curves run on different days did not differ significantly. It was sensitive (lower limit of quantitation (LLOQ) = 25.03 ng/mL), specific (the co-formers did not interfere with the determination of the drug), and robust to small changes in the mobile phase (pH, composition, and flow rate). The in vitro release of asenapine maleate from the co-crystals and the physical mixture was much enhanced when compared to the in vitro dissolution of the unprocessed drug. In conclusion, the developed and validated RP-HPLC method met the acceptance criteria and was applied successfully in evaluating the in vitro release of the drug. View Full-Text
Keywords: asenapine maleate; RP-HPLC method; validation; co-crystals; application; in vitro release asenapine maleate; RP-HPLC method; validation; co-crystals; application; in vitro release
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MDPI and ACS Style

Al-Nimry, S.S.; Khanfar, M.S. Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals. Sci. Pharm. 2021, 89, 14. https://doi.org/10.3390/scipharm89010014

AMA Style

Al-Nimry SS, Khanfar MS. Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals. Scientia Pharmaceutica. 2021; 89(1):14. https://doi.org/10.3390/scipharm89010014

Chicago/Turabian Style

Al-Nimry, Suhair S.; Khanfar, Mai S. 2021. "Validation of an RP-HPLC Method for the Determination of Asenapine Maleate in Dissolution Media and Application to Study In Vitro Release from Co-Crystals" Sci. Pharm. 89, no. 1: 14. https://doi.org/10.3390/scipharm89010014

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