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Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to l-Ascorbic Acid

1
Department of Cardiology and Vascular Medicine, Soetomo General Hospital, Faculty of Medicine, Universitas Airlangga, Mayjend. Prof. Dr. Moestopo Street No. 6-8, 60286 Surabaya, Indonesia
2
School of Management, Healthcare Entrepreneurship Division, University College London, Gower St, Bloomsbury, WC1E 6BT London, UK
3
Stem Cell Divison, Prodia Laboratory, Kramat 7 No. 11 Street, 10430 Jakarta, Indonesia
4
Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Universitas Trisakti, Kyai Tapa Street No.260, 11440 Jakarta, Indonesia
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2019, 87(3), 16; https://doi.org/10.3390/scipharm87030016
Received: 9 May 2019 / Revised: 25 June 2019 / Accepted: 28 June 2019 / Published: 3 July 2019
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PDF [1470 KB, uploaded 3 July 2019]
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Abstract

Impairment of the endothelial progenitor cells (EPCs) ability to proliferate and migrate in the patients with coronary heart disease (CHD) is partly caused by oxidative stress. This research evaluates the effect of treatment with Ipomoea batatas L./purple sweet potato (PSP) extract and l-ascorbic acid on the proliferation and migration of impaired EPCs. EPCs were isolated from CHD patient’s peripheral blood. EPCs culture were cultivated and divided into control (untreated), PSP extract treatment (dose 1 and 25 μg/mL), and l-ascorbic acid treatment (dose 10 and 250 μg/mL) groups for 48 h. EPCs proliferation was analyzed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay, and migration was evaluated with the cell migration assay kit. Statistical tests were evaluated using SPSS 25.0. This research showed that EPCs proliferation and migration was significantly higher in all PSP extract and l-ascorbic acid treatment compared to the control (p < 0.001). EPCs migration on treatment with a PSP extract dose of 25 μg/mL was significantly higher compared to the treatment with l-ascorbic acid dose of 250 μg/mL (303,000 ± 1000 compared to 215,000 ± 3000 cells, p< 0.001). In conclusion, both treatments with PSP extract and l-ascorbic acid can improve the proliferation and migration of impaired EPCs. At the dose of 25 μg/mL, PSP extract seems to be superior to the l-ascorbic acid dose of 250 μg/mL to improve EPCs migration. View Full-Text
Keywords: antioxidant; anthocyanin; coronary heart disease; functional antioxidant; anthocyanin; coronary heart disease; functional
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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MDPI and ACS Style

Oktaviono, Y.H.; Al-Farabi, M.J.; Suastika, L.O.S.; Hartono, F.; Dirgantara, Y.; Sandra, F. Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to l-Ascorbic Acid. Sci. Pharm. 2019, 87, 16.

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